Luận án Nghiên cứu thành phần hóa học của dịch trích bã hột cây bông gossypium herbaceum, bởi nước

Phl1nNghien Cliu -ChudngIV -67- ChuangIV. NGHIEN CUU PHAN DO~NCO HO~T TiNH L(1I SUA: PHAN DO~N 50KD - tOOKDvA PHAN DO~N5KD -50KD Cac ke'tlu~nd chuangIII chohie'tcacphando~ncuaeha'trichbffh<)thong deu ehuaproteinva polisaearid.MOt phan do~nco ho~ttinh IQi suaphai ehua proteinva polisacaridvdi hamlu.<fngcao.Sl{tinhchCkC tier la giai do<;tndin thiG't dCtImhiduthemvCUlc phando<;tnc6 ho<;tttinhl<jisua. C6 nhiCuphu.<1ngphapdC tinhchC,dl{atrensl{khacbi~tcuacaephanta vc kich thudc,dOhoa tantrongdungmoi, dic$nticheuaphanta, tinh ai ll{cd~ebic$t cuavailo~in6ichua trongphanta .Vdicaed~etinhneutren,nhungphuongphap tinhche'sad~ng]a:siteky IQcgel, sitcky iraQd6i ion vasiteky ai ll{e. IV .1.S~cky IQc~cl. IV.I.I.Nguyentitceuaphuongphapsiickv lac~el Gel thongd~ngco tenthuongm~i sephaeryl,sephadex,sepharoz,]amQt lo~ipolimerco nhanheuadextran.No thongtannhungra'tng~mnudc,cod~ng htnhCalliron,duongkinhtrungbinh0,1rum. Chodungdjehgelbaoh<)atrongdungmoivaocQtsitcky , tie'pdo,chom~u cha'tdaticOt. Khi giai ly, caephantaco trQngluQngphantanho, duQephanb6 trongdungm6id khoangtr6nggiuacaeh~tgel vae6thSxuyenvaon~mtrong nhungh<;ttgel.PhantaeotrQnglu<jngphanta IOnthongchilivaocaeh~tgel,d trongdungdjch,ehie'mkh6nggiangiuaeaeh<;ttgel,sedi ehuySnnhanhrakhoicQt trude.(Phant6ngquanII.8). IV.1.2.Phuongphapsiteky IQegel,dunggelSEPHADEXG-IOOcl{cmin,apd~ng trenphando~n50 - 100KD. . IV./.2.a.Thlfcnghi?m Kich thu(JccQt: chi'eucaD duongkinh thStichchung thetichtr6ng Thead6i sackj : b~ngkhucx~kG'. ThlfC nghi?m : hoa tan phan do<;tn50 - 100 KD (2g) trong nude ea't (1600). Dun Hongcachthuyvai phut.Ly tam9.000vong/ phut trong15phut.Thu duQc c~n(50 mg).Cho dungdjch vaodaticQtsitcky, dungnudeca'tlam dungmoi giai ly vdi v~nt6e0,25ml / phut.Hungeha'tdungly trongnhung6ngnghic$m.Bongco chilnkh6ngcaephilndo<;tnhudu<fc. 55em 5em 90500 28000 Phdn Nghien Cliu -Chu(Jflg IV -68- PhanHchhamluQngnitrogenvaphantichvoimaysiicky lOngcaoap,dung d)tBONDAGELE-125,v~nt6cgiiii Iy0,5mJ/phut,mQtsO'phando(;ln. Iv.f.2.h.Ketqua Ghitrongbang23 vacachlnh19,20,21. BANG 23 : Phuongphapsiicky IQcgelG - 100SF , apdl;}ngtrenphando(;ln sieuIQc50 - 100KD . HINH 19:siic ky lQcgel G-IOO,tnSnphando(;lnsieuIQc50 - 100KD ~. (.) ':J J: ~ O. Q G3 G4 G5 G6 G7 0 10 20 30 40 so 60 70 80 90 100 110 120 s6 6NG NGHI~M (8,Sml/ ring) SCJlh"' III Ten Th0 Heh, Chf'l oClngdJ flam )u'(Jng PhanHeh TT CJngnghi(:m philnoo;.ln (ml) chillI kh(lng, nilrogen ki0mIra Ohi eh" (mg) (%) 1 128 0 2 1 - 10 01 85 0 3 II - 21 02 93 37.6 4 22- 3() 03 130 95.1 4.5 voi HPLC (phandon diiuA) 5 37- 48 04 96 86.1 5.6 (phandon chanhB) () 49 - 81 05 247 734.3 8.7 voi HPLC - nt - 7 82- 107 G6 218 642.8 2.5 voi HPLC (phando1I eu6iC) 8 108- 198 07 768 21.1 - nt - 9 Rii'aeOI G8 900 14 - lit - Thu h6i toanphiill : 83,6% Ph'tinNf!.ht'enCt(u - Chu:anf!.IV -69- HINH 20:So sanhcacphando<;lncuaphu'ongphaps3:cky lQcgel G-]00 biingmay HPLC, cQtBONDAGEL E - ]25 , ph<ithit;nbiingtango<;lid 250nm. PHAN J-)O~NSIEU LQC 50- 100KD .., ... ,', "'-'- - -- - - --.,-' -'- ;,- ' , I L,t h I:' Phu ' t ., I,' " " -""'-1 ' j .. . -. . ..... /-"--'" PHAN DOAN SAC KY G3 ~> .~~ . .J \. .- ",.-, ,.,",.,.-., '.-- .- ' '---"-'-----. "--, t~I ,", (- , Phut"',. ~j ,- -- ", , I I-" . t" 'r,. I i' ", '. I I ./ \__.~"'- '"-" '.-,,'-'-- -,w"'"'- ..--'-.-.-------------------------- PHAN l:)O~NSAC KY G5 t , Phtit;. "I , ,=, ':i '.,' " ,... , " I l ~ . !'\ l-, ..' I ,'1 I I I '.",' 'I I I I \, I I I """.."'. ,.-" '--.) ~ \. l '\.. '" ".-' ',. , \_.-' -"... .-.'-- ...--.---' ---.. PHAN l:)O~NSAC KY G7 .. ~.-- " '-.- ) ,.. , Of ;') "", ':' . Phut illl : ,t :'1~.:' :: ):",'1,:1.:':I:'C 'f: t(, ' I",.." !-! l r.:'.'.'-'y"'f j -"."",';"""""""'-".I I',jr 'I'..',."_, ~,1! .. ,.', I ,,).~-"-, C'-"'~ l'r'" ..' ,,;'j 1/.,.--'1., ,..6"110'.",:;~,;'t:A. ,;,~ -- '..'1f1 I "";;il_,dLI-;,' =,~I;;'r '-, nii" If' '11 <fl. ) r~ 1~ri I 'g ",.,.'> it..~ I,J", . <,j i,., \..I~'___"-"' ""'-- - ----.-------------- Ph(Jn NghienCau - Cht/(fngIV -70- HINH 21 : Ke'tquadi~ndi cacphando<;lncuasi\cky IQcgelG-IOO clfcmill, ap dt;mgtrenphando<;lnsieuIQc50- 100KD . Di~ndi SDS-PAGE vdi T= 10-20% , C=2,7%.Ban gel 10cmxl0cm, d'ay1mm. Cu'ongde)dong di~nkhong d6i 10mA ,trong 160phut . HINH 21 '. -Z , III Il- I 1 2 3 7-"-'" 4 5 6 -/7 8 9 Cac C(lttheothu:tT!tittrai quaphdi. C(Jt 1 Chelltrichth(jD C(n 2 Phan dognsieu l(JC50 - 100KD C(lt3 ChellchuiJ'ncuahangPHARMACIA C(Jt4 PhandO(lnsackyl()cgel 03 C(Jt5 PhandognsackylQcgel G4 C(Jt6 Phandognsackyl(JCgel G5 C(Jt7 PhandognsackylQCgel G6 C(Jt8 Phandognsackyl(JCgel G7 C(Jt9 PhandO(lnsackyl(JCgel G8 l