This article reports the use of BCG, BPB and BTB
as an anionic dyes for the extractive
spectrophotometric determination of ciprofloxacin.
The performance order of the proposed methods is
BTB > BCG > BPB. No interference from common
excipients was encountered. The proposed methods
are found to be simple, sensitive, selective, accurate,
precise, economical and can be used in the
determination of ciprofloxacin in different
pharmaceutical preparations (tablets, infucsions and
eye drops).
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Vietnam Journal of Chemistry, International Edition, 55(6): 767-774, 2017
DOI: 10.15625/2525-2321.2017-00542
767
Extractive spectrophotometric methods for determination of
ciprofloxacin in pharmaceutical formulations using sulfonephthalein
acid dyes
Nguyen Trung Dung
1*
, Le Hoc Bau
1
, Le Quang Thao
1
, Nguyen Quang Dat
1
Faculty of Physics and chemical Engineering, Le Quy Don Technical University, Ha Noi, Viet Nam
Received 17 August 2017; Accepted for publication 29 December 2017
Abstract
Three simple, rapid, sensitive and accurate extractive-spectrophotometric method for the determination of
ciprofloxacin in pharmaceutical preparation has been developed. These methods are based on the formation of yellow
ion-pair complexes between the examined drug and three sulfonephthalein acid dyes, namely; bromophenol blue
(BPB), bromocresol green (BCG), and bromothymol blue (BTB) in acidic medium. The formed complexes were
extracted with chloroform and measured at 420, the colored chromogen was stable for twenty four hours. The effect of
optimum conditions via pH, dye concentration, time and solvent are studied. Beer’s law is obeyed in the concentration
ranges 0.50-25.0 μg/mL with molar absorptivity of 1.46 104, 1.83 104 and 2.07 104 L. mol-1. cm-1 and limit of
detection (LOD) of 0.105, 0.101, 0.084 for BPB, BCG and BTB methods, respectively. No interference was observed
from common excipients present in pharmaceutical formulations. The proposed method has been applied successfully to
determine ciprofloxacin in pharmaceutical preparation (tablets, infusion and eye drops).
Keywords. Ciprofloxacin, extraction-spectrophotometry, ion pair complex; sulfonephthalein dyes.
1. INTRODUCTION
Ciprofloxacin (CPF), 1-cyclopropyl-6-fluoro-4-oxo-
7-(piperazin-1-yl)-quinoline-3-carboxylic acid
(Fig.1), is a second generation fluoroquinolone
antibacterial agent with a broad spectrum of activity
against a variety of gram positive and gram negative
bacteria. It is widely used in the treatment of acute
sinusitis, lower respiratory tract infection, urinary
tract infection, chronic bacterial prostatitis and non-
complicated intra abdominal infections caused by E.
coli, P. aeruginosa, Proteus mirabilis when used in
combination with metronidazole[1].
A number of analytical methods have been
reported for the determination of ciprofloxacin in
pharmaceutical dosage forms and biological fluids
including spectrofluorometric, micellar
electrokinetic chromatography, differential pulse
voltammetry, flow injection analysis, high-
performance liquid chromatography, liquid
chromatography tandem mass spectrometry and
spectrophotometric [2-4]. Spectrophotometry is
considered as the most convenient analytical
technique in pharmaceutical analysis because of its
inherent simplicity and availability in most quality
control and clinical laboratories. Spectrophotometric
methods reported for the determination of
ciprofloxacin include oxidative coupling with 3-
methyl-2-benzothiazolinonehydrazone
hydrochloride (MBTH) and cerium (IV) ammonium
sulfate, Fe(III)- MBTH, Fe(III)-1,10-phenanthroline,
Fe(III)-Bipyridil [5-6], charge-transfer complexation
with p-acceptors such as 2,3-dichloro-5,6-dicyano-q-
benzoquinone, 7,7,8,8-tetracyanoquinodimethane
(TCNQ), p-chloranil, p-nitrophenol and
tetracyanoethylene [7], ion-pair complex formation
with acid–dye reagents such as cobalt (II)
tetrathiocyanate, Bi (III) tetraiodide, sudan III,
methyl orange, supracene violet 3B, tropeolin 00,
bromophenol blue, bromothymol blue or
bromocresol purple [8-11].
However, the disadvantages of using these
methods are that the reaction is often narrow
linearity range, requiring heating, long time for the
reaction to complete, low stability of the colored
product formed.
Bromophenol blue (BPB), bromocresol green
(BCG) and bromothymol blue (BTB) are known to
yield an ion-pair complex, which are applied in the
determination of many pharmaceutical compounds
by extractive spectrophotometric[12-13]. The
methods based on ion pair complexes extractable
into a suitable organic solvent have been shown to
be simple, sensitive, accurate and economical.
VJC, 55(6), 2017 Nguyen Trung Dung et al.
768
In this paper we report three simple, rapid and
sensitive extractive spectrophotometric methods for
the determination of ciprofloxacin in pharmaceutical
formulations. The methods are based on ion-pair
complexation between ciprofloxacin with anionic
dye namely bromophenol blue (BPB), bromocresol
green (BCG) and bromothymol blue (BTB)
subsequent extraction into chloroform and measure
the absorbance of color complex. The proposed
methods were applied to the determination of
ciprofloxacin in pharmaceutical preparation.
.
.
N
F
O
OH
O
N
HN
Figure 1: Chemical structure of ciprofloxacin
2. EXPERIMENTAL
2.1. Chemicals and equipment
All chemicals used were of analytical grade and
double distilled water was used for dilution of
reagents and samples. Ciprofloxacin hydrochloride
(Sigma-Aldrich, Germany, certified to be 99.0%),
bromophenol blue (BPB), bromocresol green (BCG)
and bromothymol blue (BTB) (Maya - R, China,
certified to be 99%) were used. The most common
solvents are chloroform, dichloromethane, carbon
tetrachloride, dichloroethane, benzene, toluene, n-
hexane and other chemicals used were of analytical
reagent grade.
The following dosage forms containing
ciprofloxacin were purchased from local pharmacy
market and employed in the study: 1 – Hasancip
and Kacipro tablets equivalent to 500 mg
ciprofloxacin (Hasan-Dermapharm and Dong Nam
manufacturing – Trading pharmaceutical Co., Ltd,
Viet Nam), 2 – Ciprofloxacin infusion equivalent to
200 mg ciprofloxacin /100 mL solution for infusion
(Hebei Tiancheng Pharmaceutical Co., Ltd and
Shandong Hualu Pharmaceutical Co., Ltd, China)
and 3 – Ciprofloxacin 0,3% eye drops equivalent to
30 mg ciprofloxacin/10 mL solution (Thanh Hoa
pharmaceutical and mediacal supplies joint stock
company, Viet Nam).
A Biochrom Model SP-60 double beam, UV-
VIS spectrophotometer (Biochrom Ltd., UK) with
1.0 cm matched quartz cells was used for absorbance
measurements.
2.2. Standard solutions
A stock solution of ciprofloxacin (1mg/mL) in
double distilled water. The working standard
solution of ciprofloxacin containing 100 µg/mL was
prepared by dilution.
The dyestuffs were used as 0.025 % solutions in
doubly distilled water.
2.3. Pharmaceutical preparations of ciprofloxacin
Tablets: Weigh and mix the contents of twenty
tablets (each one contains 500 mg ciprofloxacin), an
accurately weighed amount of powder equivalent to
0.1g of CPF transferred in to a 100 mL beaker.
Using a magnetic stirrer, the powder was completely
disintegrated in doubly distilled water, filtered
through a Whatman filter paper No 40 and diluted
up to 100 mL with doubly distilled water in a
volumetric flask. The working solution of the drug
containing 100 µg/mL was prepared by dilution and
the below procedure was followed.
Infusion solution (2 mg/mL) and eye drops (30
mg, 10 mL each ): a suitable volume was diluted to
100 µg/mL with double distilled water and the
below procedure was followed.
2.4. Procedure and calibration graph
Into a series of 125 mL separating funnel, volumes
of CPF working standard solution equivalent to 0.5-
25 μg/mL were transferred. To each funnel, add 4.0
mL of 0.025% BPB, BCG, BTB, respectively and
mixed well. Then 10 mL of chloroform was added to
each of the separating funnel. The contents were
shaken for 2 min and allowed to separate the two
layers. The absorbance of the organic phase at 420
nm was measured in each case against a reagent
blank similarly prepared and and a calibration graph
was constructed. The colored chromogen was stable
for twenty four hours.
2.5. Statistical analysis
Method was validated according to ICH
Guidelines[14], in terms of linearity and range,
accuracy and precision, limit of detection (LOD),
limit of quantitation (LOQ).
Calculation and processing of data were done
using the programs Origin Pro 8.0 (USA).
3. RESULTS AND DISCUSSION
3.1. Principles of the method
VJC, 55(6), 2017 Extractive spectrophotometric methods for
769
Ciprofloxacin contains a secondary amino
group, which is protonated in acid medium, while
sulphonic acid group is present in BPB, BCG and
BTB that is the only group undergoing dissociation
in the pH range 1-5. The colour of such dyes is due
to the opening of lactoid ring and subsequent
formation of quinoid group. It is supposed that the
two tautomers are present in equilibrium but due to
strong acidic nature of the sulphonic acid group, the
quinoid body must predominate. Finally, the
protonated ciprofloxacin forms ion-pairs with
anionic dyes, which are quantitatively extracted into
chloroform. The possible reaction mechanisms are
proposed and given in figure 2.
Figure 2: The possible reaction mechanism for the reaction between ciprofloxacin and bromocresol green,
bromothymol blue
3.2. Optimum reaction conditions for complex
formation
The optimization of the methods was carefully
studied to achieve complete reaction formation,
highest sensitivity and maximum absorbance. The
following parameters were optimized such as dye
concentration, type of extracting solvent, effect of
pH and effect of shaking time.
3.1.2. Effect of pH
The influence of pH on the ion-pair formations of
ciprofloxacin with various dyes has been studied
using HCl 1 M and NaOH 1 M. It was noticed that
the highest absorbance value were observed at pH
VJC, 55(6), 2017 Nguyen Trung Dung et al.
770
3.3, 3.4 and 3.5 for BPB, BTB and BCG method,
respectively (Fig. 3). Thus, all the absorbance
measurements were made at pH 3.3, 3.4 and 3.5 with
BPB, BTB and BCG, respectively.
1 2 3 4 5 6 7
0.0
0.2
0.4
0.6
0.8
1.0
A
b
s
o
rb
a
n
c
e
pH
CPF- BCG
CPF- BPB
CPF- BTB
Figure 3: Effect of pH on the absorbance of 10
g.mL-1 CPF acid-dye
3.2.2. Effect of dyestuff concentration
The effect of dyestuff concentrations was also
studied by adding different volumes of 0.025 %
dyestuff (0.5-7.0 mL) to a constant amount of
CPF(10 μg.mL-1). The results showed that the
maximum color intensity of the complex was
achieved with 4.0 mL of 0.025 % of each dye. Thus,
4 mL of each dyestuff was used for ion-pair
formation throughout the experiment (Fig. 4).
0 1 2 3 4 5 6 7 8
0.0
0.2
0.4
0.6
0.8
1.0
A
b
s
o
rb
a
n
c
e
Volume of dye (mL)
CPF-BPB
CPF-BCG
CPF-BTB
Figure 4: Effect of the volume of 0.025 % dyes with
10 g.mL
-1
CPF
3.2.3. Effect of extracting solvent
A number of organic solvents such as chloroform,
carbon tetrachloride, dichloromethane, benzene and
toluene were examined for extraction of the ion-pair
complex in order to provide an applicable extraction
procedure. The most convenient solvent found to
produce the highest absorbance, extraction power
and stability of color of the formed ion-associates
was chloroform for BCG, BPB and BTB (table 1).
Table 1: The effect solvent that required for ion-pair
complex formation ( max = 420 nm)
Organic solvent
Absorbance (10 µg/mL of
CPF)
BPB BCG BTB
Chloroform 0.452 0.565 0.695
Dichloromethane 0.436 0.553 0.663
Dichloroethane 0.182 0.325 0.140
Carbon
tetrachloride
0.033 0.046 0.047
Benzene 0.028 0.044 0.175
Toluene 0.133 0.008 0.074
3.2.4. Effect of shaking time
The effect of shaking time on the formation and
stability of the ion-pair complex was studied by
measuring the absorbance of the extracted ion-
associates at increasing time intervals (0-4.0 min), the
results showed that the ion-pair complex were
formed almost instantaneously in all cases at room
temperature with 2.0 min shaking time (Fig. 5). The
absorbances of the complexes were found to be
stable for more than 24 h.
0 1 2 3 4
0.0
0.2
0.4
0.6
0.8
1.0
A
b
s
o
rb
a
n
c
e
Shaking time (min)
CPF-BPB
CPF-BCG
CPF-BTB
Figure 5: Effect of shaking time on the ion
pair complexes
3.2.5. Composition of ion-pair complexes
Job’s method of continuous variation of equimolar
solutions was employed: a 3.0×10−4M standard
solution of ciprofloxacin and 3.0×10−4M solution of
BPB, BCG and BTB, respectively, were used. A
series solutions was prepared in which the total
volume of drug and reagent was kept at 10 mL for
BPB, BCG and BTB, respectively. The absorbance
was measured at 420 nm for each dye. The molar
ratio of the reagents (drug:drug+dye) in the ion-pair
complexes was determined by the method
VJC, 55(6), 2017 Extractive spectrophotometric methods for
771
continuous variations (Job’s method) (Fig. 6).The
results indicate that 1:1 (drug:dye) ion-pairs are
formed through the electrostatic attraction between
positive protonated CPF
+
and negative BPB
-
, BCG
-
and BTB-. The extraction equilibrium can be
represented as follows:
CPF+(aq) + D
-
(aq) CPF
+ D-(aq) CPF
+ D-(org)
where CPF+ and D− represent the protonated
ciprofloxacin and the anion of the dye, respectively,
and the subcript (aq) and (org) refer to the aqueous
and organic phases, respectively.
0.00 0.25 0.50 0.75 1.00
0.0
0.2
0.4
0.6
0.8
1.0
A
b
s
o
rb
a
n
c
e
[CPF]/[CPF+dye]
CPF-BPB
CPF-BCG
CPF-BTB
Figure 6: Job’s method of continuous variation
graph for the reaction of ciprofloxacin with acid-
dyes BPB BCG and BTB, [drug]=[dye] = 3×10-4 M
3.2.6. Spectral characteristics
The absorption spectra of the ion-pair complexes
extracted into chloroform are shown in Fig. 7. The
ion-pair complexes with BTB, BPB and BCG
absorbed maximally at 420 nm. The reagent blank
under similar conditions showed no absorption.
360 400 440 480 520
0.0
0.2
0.4
0.6
0.8
1.0
A
b
s
o
rb
a
n
c
e
Wavelength(nm)
BPB
BCG
BTB
CPF-BPB
CPF-BCG
CPF-BTB
Figure 7: Absorption spectra of ciprofloxacin
(10µg.mL-1)-dye complex extracted into 10 mL
chloroform
3.3. Validation of the proposed method
The proposed methods are successfully validated
according to International Conference on
Harmonization (ICH) guidelines [14]. The limit of
detection (LOD) and quantification (LOQ) of the
method are given by and respectively,
relative standard deviation (RSD(%)= ;
where SD is the standard deviation of blank
absorbance values, b is the slope of the calibration
curve equation, " is the average value of the
measurement. Blank samples were prepared as
described in section 2.4 but without CPF.
Under the described experimental conditions,
calibration curves for proposed methods were
constructed (Fig. 8). The linear regression equations,
standard deviation, slopes and intercepts, correlation
coefficients, relative standard deviation of response
factors, and linearity ranges were given in (table 2)
for each proposed spectrophotometric method. The
molar absorptivities, Sandell’s sensitivity of each
methods was calculated and these values showed
that the molar absorptivity of BTB > BCG > BPB
ion-pair complexes.
Figure 8: Standard curves of CPF ion pairs with
BTB, BCG and BPB at max 420 nm
The accuracy and precision of the methods were
determined by preparing solutions of three different
concentrations of ciprofloxacin and analyzing them
in six replicates. Samples for analysis were prepared
as described in section 2.4. The precision of the
proposed methods was evaluated as percentage
relative standard deviation (RSD%) and accuracy as
percentage relative error (RE%). The percentage
relative error calculated using the following
equation: RE(%) =[(founded–added)/ added]x 100
The accuracy and precision results are shown in
table 3.
The low values of the relative standard deviation
percentage and relative error percentage specify the
high precision and the good accuracy of the method.
VJC, 55(6), 2017 Nguyen Trung Dung et al.
772
Table 2: Analytical characteristics of the proposed methods (n = 6)
Parameters
Proposed methods
BPB BCG BTB
Colour Yellow Yellow Yellow
Wavelengths λmax (nm) 420 420 420
pH 3.3 3.5 3.4
Stability (h) 24 24 24
Shaking time (min) 2 2 2
Stoichiometric ratio 1:1 1:1 1:1
Beer’s law range (µg.mL-1) 0.5-25 0.5-25 0.5-25
Limit of detection (µg.mL-1) 0.105 0.101 0.084
Limit of quantitation (µg.mL-1) 0.315 0.303 0.252
Molar absorbitivity (L.mol-1.cm-1) 1.46x104 1.83x104 2.07x104
Sandell’s sensitivity (µg/cm2) 0.0686 0.0562 0.0462
Regression equation (Y = bx +a)
Slope (b) 0.0441 0.0552 0.0625
Intercept (a) 0.0053 0.0287 0.0762
Correlation coefficient (R2) 0.996 0.998 0.997
Table 3: Evaluation of accuracy and precision of the proposed methods (n = 6)
Method
Amount taken
( g.mL-1)
Amount found
( g.mL-1)
Recovery
(%)
RSD (%) RE (%)
BCG
5.00 5.08 101.6 1.16 1.60
10.00 9.97 99.70 0.48 -0.30
15.00 14.84 98.93 0.75 -1.07
BPB
5.00 5.06 101.2 0.97 1.20
10.00 10.08 100.8 0.54 0.80
15.00 14.94 99.60 0.86 -0.4
BTB
5.00 4.94 98.80 1.02 -1.2
10.00 9.96 99.60 0.57 -0.4
15.00 15.18 101.2 0.94 1.2
3.4. Effects of interference
The extent of interference by various excipients
(magnesium stearate, glucose, lactose, starch and
sodium chloride) which often accompany the
pharmaceutical preparations was studied in a total
volume of 10 mL chloroform. The interference was
determined by measuring the absorbance of a
solution containing 10 µg/mL of ciprofloxacin. This
study was carried out by following the proposed
procedures for a 10 mL sample system, by adding a
known amount of foreign species to a ciprofloxacin
solution of 10 µg/mL. The tolerance limits of
interfering species were established at those
concentrations that do not cause more than ±2.0%
error. The tolerance limits of excipients are listed in
table 4. The results indicated that there is no
interference from the degradation, indicating a high
selectivity for determining the studied ciprofloxacin
in its dosage forms.
Table 4: Effect of foreign species on the
determination of 10 µg.mL-1 ciprofloxacin
Excipients
Tolerance limit
(μg. mL-1)
Magnesium stearate 500
Glucose 250
Lactose 500
Sodium chloride 500
Starch 250
3.5. Comparison with other spectrophotometric
methods
The proposed method compares favorably with other
reported methods. As shown in table 5 the proposed
method is more high sensitivity than other methods,
VJC, 55(6), 2017 Extractive spectrophotometric methods for
773
needs no heating, the product is stable for a longer
time and and are free from interference with
common excipients.
Table 5: Comparison of VIS spectrophotometric methods for ciprofloxacin determination
No. Reagent max\
(nm)
Range of
determination
( g. mL−1)
Molar
absorbitivity
(L.mol-1.cm-1)
Remarks Ref.
1 Ce(IV)- MBTH 630 10-50 -
Involves contact
time
[5]
3
Fe(III)-1,10-
phenanthroline
510 0.04-7.2 3.4 104
Involves contact
time and heating [6]
4 Fe(III)-Bipyridil 522 0.05-9 2.95 104
Involves contact
time and heating
5 CL 520 16-96 -
Involves contact
time and heating
[7]
6 TCNE 335 0.25-15 -
Involves contact
time and heating
7
Co(II)
tetrathiocyanate
623 20-240 8.38 102 Less sensitive [8]
8 Sudan III 566 0.4-10.4 2.38 104
Involves heating
/cooling samples
[9]
9 Eosin Y 547 2-8 3.56 104
Less stable colour
[10]
10 Merbromin 545 2-15 1.23 104
11 BPB 420 0.5-25.0 1.46×104 Short reaction
time, high
sensitive and high
colour stability
This
work 12 BCG 420 0.5-25.0 1.83×104
13 BTB 420 0.5-25.0 2.07×104
MBTH: 3-methyl-2-benzothioazolin-2-one-hydrazone; CL: p-chloranil; TCNE: Tetracyanoethylene; BPB:
Bromophenol blue, BCG: Bromocresol green and BTB: Bromothymol blue
3.6. Application of the proposed methods
The proposed method was successfully applied to
determine ciprofloxacin in different pharmaceutical
preparations (tablets, capsules and eye drops). The
results given in table 6 of the analysis showed that
the data are consistent with the label claim of the
formulations. The relative standard deviation values
are below 2 % indicating the precision of the
method. The validations of the proposed methods
were further confirmed by recovery studies. The %
recovery vary from 97.41 to 101.20 indicating high
accuracy of methods.
Table 6: Results of ciprofloxacin determination in pharmaceutical preparations
Pharmaceutical
preparation
Labeled amount
(mg/form)
Recovery (%) RSD (%)
BCG BPB BTB BCG BPB BTB
Hasancip tablet 500 mg /tablet 99.75 99.36 98.89 0.59 0.28 0.16
Kacipro tablet 500 mg /tablet 98.36 98.57 101.20 0.74 0.45 0.24
Shandong
infusion
200 mg/100 mL 102.53 100.54 97.41 0.43 0.32 0.26
Hebei infusion 200 mg/100 mL 98.05 101.08 97.69 0.87 0.65 0.48
Eye drops 30mg/tube 100.24 99.02 98.53 0.78 0.51 0.39
VJC, 55(6), 2017 Nguyen Trung Dung et al.
774
4. CONCLUSION
This article reports the use of BCG, BPB and BTB
as an anionic dyes for the extractive
spectrophotometric determination of ciprofloxacin.
The performance order of the proposed methods is
BTB > BCG > BPB. No interference from common
excipients was encountered. The proposed methods
are found to be simple, sensitive, selective, accurate,
precise, economical and can be used in the
determination of ciprofloxacin in different
pharmaceutical preparations (tablets, infucsions and
eye drops).
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Corresponding author: Nguyen Trung Dung
Faculty of Physics an chemical Engineering, Le Quy Don Technical University
236, Hoang Quoc Viet road, Cau Giay district, Hanoi, Viet Nam
E-mail: nguyentrungdung1980@gmail.com.
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