NHÂN GIỐNG VÔ TÍNH MỘT SỐ LOÀI LAN HỒ ĐIỆP
(PHALAENOPSIS SP.)
Võ Thị Bạch Mai
Trang nhan đề
Mục lục
Mở đầu
Tổng quan
Phần nghiên cứu
Thảo luận chung
Kết luận và đề nghị
Phụ lục
Tài liệu tham khảo
M£lC [£lC Wn An PTS. Khoa Hqc
MQCLVC
P DAN l\tl{j DAU
1. Mt;lc dlch nghien cU"u 1
2. Thanh t1fukhoa hQc va ynghIa tln1c ti~n 2
~ ~
PHAN TONG QUAN
1. Khai nit%mchung v~ sinh tntdng dinh duong 4
2. Ngu6n g6c va d~c tinh sinh tnfdng-pbat triSn cua cay Ian H6 Bi~p 4
2.1. Stf phan b6 4
2 ")
C
"" " . 6 .,. au t~o cua cay
2.2.1. Cd quail dinh du'ong 6
2.2.2. Cd quail sinh san 8
,.,2 3 S h ~ 'K " .,. . . tf p at tnen cua cay . 11
2.2.3.1. NhfiTIgd~c tlnh v~ giai do~n phat triSn cua trvc phat boa 11
2.2.3.2. Stf tr6 hoa 14
"', , V. "",
"':'.-'. l tn cua pohng Ian H0A:nu'l"t(P tre"n thi. tru'';uvng .1 6
3. i\nh huang cua chftt BHSTTV va mQt sO'cha't khac tren stf sinh tnidng
va phat trien 17
3.1. Lich 51.1' 17
3.2. Thu~t ngu 18
Mlj-C llj-c Lu4n An ITS. Khoa Hqc
3.3. Ca'u truc va chU'cDang ho~t dQng 19
3.3.1. Auxin 19
3.3.2. Giberenlin . , 20
3.3.3. Xitokinin ,. . 21
"3.3.4. Axit apxixic 22
3.3.5. Etylen 23
3.4. .O-phenyl hydroxylamin 23
3.5. HQpcha't phenol va cac cha't U'cche'-sinh trltang khac 24
3.6. Lanolin 24
4. Vai tro sinh ly cda rnQts6 nguyen t6 khoang 25
4.1. Nitd (N) 25
4.2. Photpho (P) 26
4.3. Kall (K) 26
4.4. Manhe (Mg) :. 27
4.5. Kern (Zn) 27
5. Sd lu'Qcv~ cac nghien cU'unhan gi6ng in-vitro 28
5.1. Nuoi d{y dinh sinh trltdng ngQnva dinh sinh trltdng nach la 28
5.2. Nuoi ca'y rn~rn ngd d nhanh phat hoa 29
5.2.1. Phu'dng phap khong qua giai do~n t~o ti~n cd 29
5 2 " Nh.~ . .-. ung nhif!:!1. ntvIt. an h hu'd.ng dclt;llnuO" !. ca")'',"r.oam ngu "9
a. Anh huang cua vi tri m§m va ky thu~t la'y m§u 29
b. Anh huang cua loai 30
c. Anh huang cua thanh pIlau khoang trong moi tru'ong nuoi ca'y.30
MIfC LIfC Lu4n An PTs. Khoa Hqc
d. Anh huang cua cha't di~u boa sinh tru'dng th11cv~t 30
e. Anh huang cua nhi~t dQva ant sang 31
f. Anh huang cua hQp cha't hUll cd phU'c tC;lP 32
5.3. Nuai ca'y doC;lnla 33
5.4. Nuai ca'y tC;lokh6i ma (cal) 33
6. So luQc nghien cU'unhan gi6ng va tint in-vivo 34
6.1. 511ph:it tri€n cua dinh sinh Wang d nach la 34
6.2. S11ph:it tri€n 'cua mftm ngii tren ph:it hoa 34
6.2.1. Anh huang cua nhit$tdQva th~i gian chi~u sang 35
6.2.2. Anh huang cua cha'tBHSTTV ngoC;lisinh 35
PuAN NGmtN c(fu
A " . "" "A ""
Phftn Mot: NGHIEN COD CAI TIEN PHUdNG PHAP NHAN GIaNG.
VO TINH IN-VITRO.
1.Md dftu . 37
2. V~t li<$uva phU'ongphap 38
2.1. V~t li<$u 38
2.2. Phuong phap 39
2.2.1. Nuai cf{ydoC;lnmang mftm tren phat hoa 39
2.2.2. Nuai c[{ydinh ngQnch6i hoa 40
2.2.3. Nuoi cffycae bQph~n cua dlY Ian con 40
2.2.4. Kha nang tC;lokh6i ma va tt~isinh kh6i mo 41
Mlfc LlfC Wn An ITS. Khoa Hqc
~
') ') 4 1 C ' b"., d !" ; hlnh th ,. . . h ';: 4 ')
., ac len OJve a1 gla1 p au , -
') ') 4 ') C ' - b"., d6'" ; , h I , ,.,.' """ ac len 1ve SID y cua rno cay 4.3
a. Do ham lu'<;Jngdi<$pl~c t6 43
b. Do cu'CJngde?qnang h<;Jp 43
c. Do 811thay d6i ham 1u'<;Jngprotein 43
3. Ke't qua va nh~n xet , 44
Ph~n Hai: NGIDEN-CUu NH.AN GIONG VO TfNH IN-VIVO.
J:: - 1.Md dan 60
2. V~t lic%uva phu'dngphap 61
2.1. V~t li<$n 61
2.2. Phu'<1ngphap . 61
2.2.1. Quan sat hlnh thai giiii phan 61
2.2.2. Do ham lu'<;Jngcha'tDHSTTV nQi sinh c6 trong phat hoa 62
2.2.3. Xu ly cha'tE>HSTTVteen cac vi tri mAmngu 64
2.2.4. XU'ly dung dich dinh du'CJng 65
2.2.5. Theo doi cac bie'n ddi sinh ly trong qua trlnh hlnh thanh v~
phat tri~n cay con 66
a. Cu'CJngdQ qnang h<;Jp 66
b. Cu'CJngdQho ha'p 66
c. Do ham 1u'<;Jndg~m t6ng s6 teen cay m~ 66
d. Do ham 1u'<;Jnaguxin nQi sinh trong cay con 66
Mlfc llfC Lu4n An PTS. Khoa HQc
2.2.6. Theo doi slf tang tru'dng va phat triSn cua cay 66
3. KSt qua va nh~n xet ; 67
PHAN THAOLU~N CHUNG
1. Anh hu'dng cua h~p cha"tphenol teen s11tang tru'dng va phat trien
7( " cua man v~t ca,.(y' 81
2. Vai tro cua cha"tDHSTTVireDs11tang tru'dngvaphat tri~n
~ 82 cua mam ngu . .
3. Kluinang t~o khc5imo va iai sinhkh5i mo 89
4. Vai tro cua cha"tkhoang trongmoi tnfOngdinh du'6ng 90
PHAN KtT LU~N VADE NGfij 94
TAl LItU TRAM K,HA0
1. Tai li~u tie'ng Vi~t Nam ~ 101
2. Tai li~u tie"ngAnh 103
3. Tai li~u tie"ngPhap 109
4. TAili~u tie'ngDd'c 112
MIfC llfC " Wn An PTS. Khoa Hqc
pRAN PHT.)Ll)C
. Moi tru'dng MS cai tie'n l13
. Moi tntdng Knudson C cai tie'n 114
. Moi tru'dng Heller 115
. Tinh th5ng ke srf gia tang sinh kh5i mo 116
. Tinh th5ng ke ham lu'Qngd~m t6ng s5 118
CAC CONGTRINH CONGB6 LItN QUANnfN LU~N AN 120
#
CACemf VIETTAT
AAB : Axit apxixic.
AlA : Axit indol axetic.
ANA : Axit naphthalen axetic.
Aux. : Auxin.
BAP : Benzyl-aminopurin.
(Cae eha't)DHSTTV : (Cae eha't) di~u boa sinh trn'ong thlfe V?t.
Ch6i DD : Ch6i dinh du'ong.
CDHH : -Cu'dngdQho ha'p.
CDQH : Cu'dng dQQuangh<;1p
Ch6i SS : Ch6i sinh san.
CSV : CQngsrivieD.
2,4 D : Axit 2,4-diclorophenoxy axetic.
FeEDTA : Ferie Etylen diamintetra axetat.
GA3 : Axit giberenlie.
Gib. : Giberenlin.
2iP : Isopentenyladenin.
KTT : Kleh Weh to'.
MS : Murashige va Skoog.
PBA : N-Benzyl-9-(2-tetrahydro~pyranyl) adenin.
PT : Phtit tri~n.
SLTV : Sinh ly thtfe V?t.
Xito. : Xitokinin.
1
Luin An PTS. Khoa HCJC
I
.,
DANH ]\1{)C ANH
1. va 2. Slt phong phil v~ hlnh d~ng va man s~c cua phong Ian Hf>Bi~p.
3. Chbi dinh duang phat tri6n tll'mc1mngli cua phat boa.
4. Ch5i sinh san phat tri~n tll'mftm ngu cua phat boa.
5. 811t~o ti~n cn (protoconns) 0 than cay con.
6. 811phM tri~n cna ch5i con 0 phftn cno'i 1a trong moi tnt~ng N2.
7. 811t~o ti~n en tll' la.
8. Kho'i mo 0 go'c cay con in-vitro.
9. Ph~u thd'c c~t ngang than vi1ng t~o kho'i mo.
10. S11gia tang sinh kho'i cua kho'i mo trong moi tnt~ng MP2.
11. Kho'i mo tcHsinh (a) va kh6i mo chu'a tm sinh (b)
12. Binh m~m xu!t hi~n 11)' kho'i mo.
13. Ph~u thd'c 'cAtngang kh6i IDOc6 nhuQIDI2K! (thu6~ nhUQIDLugo1).
14. Sd dB ph~u thd'c cAtngang wng t~o r~.
15. 811tcii sinh cay con to' kh6i mo.
16. 811t~o r~ 0 cay con in-vitro.
17. L1;lcl~p trong tt bao cna phcit boa.
18. Ch5i dinh du'ong va ch5i sinh san xua't hi~n san khi xu 1:9h6n h<;lpB6.
19. Cay con phM tri~n tU'ID~IDngd cua phat boa.
20. Cay con phat tri~n c6 d~y dti 1a va r~.
21. Cay ra boa l~n d~u.
~ Lu#.n An PTS Khoa HfX
, "
DANHM1)C CACBANG
1. Nuoi cfiy do~n mang m~m ngu tren phat boa d 28°C.
2. Nuoi cfiy do~n mang m~m ngti tren phat boa d 20DC.
3. Nuoi cfiy dinh ngQnm~m ngu phat boa san 1 thang.
4. Ti l~ (%) m~u cfiy phat sinh cd quail va d~c tinh cua cac bQph~n mdi
du'<;jc~o thanh san 8-10 tu~n.
5. Anh hu'dng cua lac dQng cd hQCdtn sf! t~o kh6i mo St;o.
6. Anh hu'dng cua n6ng dQchit DHSTTV d~n sT!gia tang sinh kh6i cua
kh6i mo.
7. Ham lu'~g di~p IQcto' cua kh6i mo.
8. Cu'CYng dQquang h<;jpcua kh6i mo.
9. Ham 1u'<;1ng proteiDe cua kh6i mo.
10.ST!tai sinh cua kh6i mo.
l1.ST!bi~n d6i ham lu'<;jngcac chit DHSTTV tren cac vi tri m~m cua nhanh
phat boa dii n~hoan toaD.
12.Sf! bi~n d6i ham lu'<;jngcac chit DHSTTV tren cac vi tri m~m cua nhanh
phat boa chu'and.
13.A.nh hu'&.g cua ndng dQ IITT tren cae m~m ngu cua phat boa san 2 tu~n.
14.CtfCYngdQQuang h<;1pcua cae vi tri m~ san khi ttfdi dinh du'(jng 1 tuAn.
15.CtfCYngdQho ha'p cua cac vi tri m~ san khi ttfdi dinh dtmng 1 tu~n.
16.Ctf(Jng dQquang h<;1pva ctf(JngdQho hip cua cac vi tri mAm san khi t~di
dinh du'CJng2 tu~n.
I
Luj.n An PTS Khoa Hr;x:
17.Ham ht<;lngd<:imtbng sr;.
18.Su' phM triSn cua c::icm~m ngu saD khi xu ly KTT. 2 thang.
19.Ham lu'<;lngauxin nQi sinh d ch6i con.
20. SIt tang tru'dng va. phat triSn cua ch6i con sau 4 thang.
,
Lu?n An PTS. Khoa H9C
,,? ~
DANH M{)CBIEUBO.
1. BiSu d6 tang tru'dng va phat tri~n cua phat hoa H6 E>i~p.
2. 811bie'n d6i ham 1u'<;1ng cae chit DHSTTV tren cae vi tr! mgm cua phat
boa dii n(1.
3. S11bie'n d6i ham lu'<;1ngcae ch[t DHSTTV trel1 cae vi tri mgm cua phM
boa chu'a nO'.
4. Cu'ClngdQ qual1g h<;1pcua cae vi tri mfun san khi tu'di dd. dinh du'ong 1
~ luau.
5. Cu'Ol1gdQho hip cua cae vi tri mgm san khi tu'di dd. dinh du'ong 1 tugn.
6. Cu'ong dQquang h<;1pva cu'ClngdQho hip cua cae vi tn mgm san khi tu'di
dd. dinh du'ong 2 tugn.
7. Ham lu'<;1ngdqm t6ng s6 tren cay my san khi tu'di dd. dinh du'ong 2 tugn.
8. Han11u'<;1ngauxin nQi shih d ch6i con.
Lu~n An FrS. Khoa H(,Jc
DANH l\1T.JCHlNH
1. Ban do phan be>khu \lIfc trong Ian Ho Di~p.
2. Ca'u ti;lodinh sinh lru'dng cua Ho £)i~p.
3. Hlnh thai ben ngoai diy Ian Ho Di<%p.
4. So do ph~u thac c~t ngang m~m d vi tri 1 va 2.
5. LM c~t dQc vi tri m~m sf) 3 va 4 tren phM boa (ch6i dinh du'ong)
6. LM c~t dQc vi tn m~m sf) 5 va 6 tren phM boa (ch6i sinh san)
7. Qui trlnh nhan gi6ng va tint Lan H6 Bi~p.
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Phin NghienCztu LuirJ anPT5. Khoa hrc
PhAnl\tl(}t:NGmtN c(fu CM TIEN PmfdNG PHAP NHAN GI6NG
vb riND IN-VITRO.
1.Ma DAV.
PhongIanla mQtrongnhffngcaycanhc6giahi kinht~caD,giahi
n§yc6lienquaild~nd:;ictinhvakhaDangnhangiO'ngcuachungnhu'kh6
ngym§m,thCligiansinhtru'dngdai,khaDangnhangiO'ngvatinhtha'p,kh6
nhangiO'ng D€ t~osO'Iu'QngIoncayconvo tinhthongthu'CJngtrongkY
ngh~sanxua'tIanngu'Clitanuoica'ydinhsinhtru'dng(Morel, 1964;Huges,
1981;Bouriquetva CSV, 1982)[83,28, 17],da du'Qcthlfchit%ntren
Cymbidium(DiaIan)vaCattleya.
Tuy nhien,dO'ivoiphongIanH6 Dit%p,trongdi~ukit%nthienlimen
chungkh6ngych6iconvat~okeikisblnhthu'Clngnhu'nhffngloaiIankhac
vahit%uquatrongnhangiO'ngvotinhin-vitrocontha'p(BouriquetvaCSV,
1982)[17].
Do d6,trongph§nnghiencoon§y,chungWi tlmcachcaitie'nkha
DangnhangiO'ngillQtsO'loaiIanH6Dit%pbAngkythu~tnuoica'yin-vitro.
-37-
Pl1iIlNgl1jenCzfu Lui.n anPTS. Khoa h9C
2.VAT LIEU vA PIRJ'ONGPIIAP.
I
") 1 'T"t I""I -.. ~ I~u.
CayPhongIanH6 Di~p(Phalaenopsjssp.)(Hlnh3) la lo~icaydon
tn;1C,s5ngbi~usinh,thuQc:
Nganh:Hi~nhoaBi tu.
Ldp:Dontudi~p.
BQ:Orchidales.
HQ:Orchidaceae.
Till nghi~mdu'Qcthtfchi~ntrenphathoa cayIan H5 f)i~p,co chiSu
daitll'40de'n50cm,ph§ndu'diphathoacotrungblnh.tll'4 de'n5 d6tco
mangm§mngu(vi tn m§ms61ddu'diclingcuacQngphelthoa),benireD
cohoadand.
V11ng!loa$li <:;"'('
VulIg cht>itkll clf'c.:
Vi1ng m~1mIlgll
IDnh3.HlnhtheiibenngoaicayIanH5f)i~p(Phalaenopsis)
-38-
Pha'nNgmen Cd'u Lujn anPTS. Khoa h9C
2.2. Phtfdngphap.
Tll'cacphatboatren,do~nmangn1~n1ngiiduQcc~tng~n,cacm§u
nftydu'QcIaub~ngc6n70°.Sail d6du'Qcruatrongdungdichc6 chuavai
giQteepoI,ruam§uvdinudcs~chvakhITtrilngtrongdungdichhypoc1oric
canxi7%trong10phut,rual~ivdinudcca'tvotrilngtll'3dSn4 Iftn.Trong
tnca'yvo tnlng,cacdo~nlien duQcc~tcachdftulien va oftududimang
mftm1 cm,tachy§y Ia baamftm.Sail d6,m§udu'Qcf{ytrongcacmoi
tru'ongdinhdu'ongva d~tnuoid 2 di~ukit$nnhit$tdQIa 20°Cva 28°C,
chie'usang16giCJ/ngay,~mdQ75-80%.
2.2.1.Nuoi C6~7do~nmangmAmtren phathoB.
Moi tru'ongdinhdu'ongSITd~ngIa MSb va MSb 1/2,thanhph~nnhu
san:
. MSb: E>aluQng,vi Iu'Qng,FeEDTA cuaMurashigeva Skoog(1962),
VitamincuaMorelvaWetmore(1951),20g/lit du'CJng,8g/litagar.
. MSb 1/2:Chi giamdaIu'Qng,vi luQng,FeEDTA cuaMS phannll'acon
cacthanhphftnkhacgill'nguyen.
Cac moi tru'CJngireDco ho~ckhongco them2,5mg/l BAP. Moi
tnfongdu'QckhITtrilngb~ngn6iha'pvo trilngd 120°C,apsua't1,2kg/cm2,
thaigian25phut.
E>€giatanght$s6nhangi6ng,chungtoi dungmoitru'CJngklchWch
slft~och6isinhsan,dStll'do co thSc~tthanhnhi~udo~nnhaco mang
-39-
PhJn Nghien Czi'u Lu~ anPTS Khoah9C
m~m.Till nghi<$mdU\iCthtfc hi<$nd 20°C,dungmoi tru'bngdinhdu'ong
MSb1/2vathayd6in5ngdQcha'tBHSTTV BAP nhu'san:1;2,5;5; 10
mgll.~ tninhhi<$ntu'qngh6anailmoi tru'bngnuoica'y,lam anhhu'dngde'n
sr!phattri~ncuamgm,chungtoi d~tmftucffyd vi tringu'qc(tITCmgm
hu'dngxuo'ngtheochi6unghich).Sankhim~mtangtru'dng1emthlcffyl~i
theochi~uthu~nblnhthu'bng.
2.2.2.Nu6ica'ydinh ngQnch6iboa.
Cae do~nmangm~mngii ciia phat boa du'qckhu trUngnhu'dii kS
tren.
Trong,tueffyYOtrung(Hottesterile),dungkypnhQntachcaey§y la
baoquanhm~mboa,chichU'a2hibaaquanhdinhm~m.
Moi tru'bngnuoicffydinhngQng5m:
. Ml:
. M2:
MSb 1/2
MSb 1/2
+0,5 mg/1AlA +0,2 mg/1kinetin.
+0,34mg/1GA3 +0,02mg/12iP.
. M3: KnudsonC +0,5 mg/12,4D+0,5 mg/1BAP.
Tit eall10itru'bngtn~nd6uc6themnu'dcdU'aydi ti 1<$2,5%.Cac blnh
caydu'cjed~tnuoi (j cungnhi<$tdQ25°C,chiSusang16giblngay,cu'bngdQ
anhsang2500-2600lux.
2.23 N "'°'" , b" h" ~ ... I.. DOlcaycae QP ~ncuacay an con.
Nhfi'ngcaycannffym~mto'h~teuagio'ngV.25909c65-6thangtu6i
ho~e68-9tu6iydi 1-2la. Cacla du'didu'qc~tthanh3 do~n(cuo'ngla,
giiYahi,ch6pla).Caemftucffydu'qenuoitrongmoitru'bngsan:
-40-
Phin Nglllen CzJu Lu?n in PTS. Khoa h9C
. N1: Heller +0,5mg/l2AD+0,5mgllBAP.
. N2: MSb 1/2+0,5mg/l2AD +0,5mgllBAP.
Cac blnhcayduqcd~tnu6id clingnhi~tdQ25°C,chie'usang16
gid/ngay,cu'dngdQanhsang2500-2600lux.
2.2.4.Khii nangt~okh6imovahii sinhkhoimo.
. SlftqOkh6imo.
Cac cay concuagiO'ngP.amabilisva P.chamonix(Antarticx
Spitzberg),trongnuoicffyin-vitrocodQtu6i4dtn5thang,caycokhming
2-3la, chu'axuffthi~nr~.
Trongmoitru'dngvo trung,dlingkim nhQndamvaog6ccuacaccay
conkhmlng4 de'n5 vi tri (phu'dngphapkichthichcdhQc).Caccaydu'Qc
cffytrongmoitru'dngMS, 1962.H6nhqpcha'tdi€uboasinhtrltdngthlfcV?t
duQcSITd:Qngtrongmoitru'dngnuoicffynhu'san:
. MSBO:0,5mgllAlA + 1,0mg/lBAP.
. MSB1:0,5mgllAlA + 2,5mg/lBAP.
. MSB2: 0,5mgllAlA + 5,0mg/lBAP.
. MSB3: 0;5mg/lAlA +10,0mg/lBAP.
. MSPO:0,5mg/lAlA + 1,0mg/lPBA.
. MSP1:0,5mg/lAlA + 2,5mg/lPBA.
. MSP2: 0,5mg/lAlA + 5,0mg/lPBA.
. MSP3: 0,5mgllAlA +1O,OmgllPBA.
-41-
Pl1/nNghitSllCzi'u Luiu tinPTS Khoa hex:
Cac blnhnuoidiy du'Qcdi;itrong2 diSukit%n:
Nuoi cayco chie'usang:cu'dngdQanhsang2000-2500lux, thdi gian
chie'usang8 gid/ngay,nhit%tdQtrungblnh 26°C :t 2°C, gmdQtrungblnh
80%.
Nuoi caytrongt6i:nhit%tdQvagmdQgi5ngnhu'diSukit$ntren.
S11gia tangsinhkh5i cuakh6i mo du'Qcxac dinhb~ngphu'ongphap
cantr~mglu'Qngcuakh6imotrongnhifngkhoangthdiglaDxacdinh.Thi
nghi~mdu'Qcb5tri ng~ulimenvdi 3 l~nli;iplqi, m6il~n3 blnhchom6i
nghit%mthuc.Ke'tquadu'QCphantichb~ngphu'ongphapphannch phu'ong
sai vdi bQ chu'ongtrlnh STATGRAPHICS, phien ban 7,0 (Statistical
GraphicsCorporation,1993).
-$-S11tM sinhcuanhifngkh5imothanhcay.
Nhifngkh6i mo chi;it,du'Qc Atra thanhnhifngkh6i vuongnha
khoang0,4- 0,5cm2vaca'yvaomoitru'dngcobancuaMS cothemANA,
(jcacndngdQ:0,5; 1,0; 1,5;3,0; 5,0mg/l.
Di;itcacblnhnuoica'yd cu'dngdQanhsang2000-2500lUX,thdi gian
chi€u sang8 gid/ngay,nhit$tdQtrungblnh 26°C::!:2°C,£m dQtrungblnh
80%.
2.2.4.1.Caebie'nd6i v~hinh thai gimphlu.
NhuQmph~uthuccAtdQcvangangvungtqokh5imovavungtqor~
(Anh13,Anh 14)b~ngthu5cthli'Lugol(h KI) vathu5cthli'Shiff.
-42-
Ph/lIlNghjeIl Czi'u Lu?n anP7S. Khoa h9C
.,.,4 ., C ' b' '" d,:', J::: , h I ' 'I ,,'""" ac len 01ve SIn y cuarnoca)7.
a.Do hamlu'dngdieDluc to':
Dungmethanoltuy~td6idS'trichdi~plQcto'.Do m~tdQquangOD d
652nmva 602TIm.Tinh hamlu'Qngdit$plQct6theocongthU'c uaBruinsm
vaHolden[1].
b.Do cu'C1ngdo Quanghdp:
Cackh6imovakh6imotai sinh,du'QcantrQngIuQng0,5g va dS'
vaodlapetritraid~u,saochocackh6imonh~nant sangtntctie'ptrong
vang3-4giC1.Tie'ntanhdocu'angdQquanghQpbhngmayWarburg,xac
dtnhlu'QngO2thayra/ddnvi thaigian/ddnvi trQnglu'Qngtu'divdi dungdich
dt$mcacbonat..
c.Do SITthayd6ihamlu'dngproteintrenmova motai sinhbhngphu'dng
phapdungaxiltricloroaxetictheoGreen'\laId [6].
Canchinhxac109mftuv~tdanghi~n huy~n,chovaobinhn6nvdi
nu'dca'tvU'adli75mI.D~yn~pblnhbhngm~tklnhd6ngh6,dunsoicach
fullY30phut,thinhthoanglqi l~c.Sail kill dS'nguQichovao dichchie't
khoang25mlaxiltricloroaxetic10%dS'thS'tichloanbQIa 100ml c6n6ng
dQaxiltricloroaxetlcla 2,5%.DS'quadem,lQcquagiffylQcvarU'ake'ttlia
b~ngaxiltricloroaxetic2%.DS'raoke'tilia,chokSttliaproteinvagiffylQC
V3.0blnhKendanvatie'ntanhdint IuQngnitdtheophudngphapKendan.
-43-
PbJn IVghjen Cd'u Lu?n in PT5. KhoabllC
3. KET QUAvA NIlANxET.
Cac do(;lnmangIn~n1ngu du'Qckhli'tri1ng(xemtrongml}c2.2), san
d6 ca'yVaGmoi tru'crngMSb c6 them2,5mgll BAP ho~cthong c6 BAP.
Ke'tquadu'QCgill nh~ntrongbangsan:
BangJ,. Nuoi cffydo(;lnmangm~mtrennhanhphatboa(j 28°C.
24569: (Jivre ( Concordex Spitzberg)
24799: Tarragone(Polar Gnll x Sonja)
24963: Chamonix(Antartiex Spitzberg)
KSt quatrenehothffytI l~manv~teffyvotrilngFJiOang74%.Man
v~teflybiiu hi~n2 d:~mghlnh :mankhongphattriin va eh6idinhdu'ong.
. Khi thongsudl}ngBAP khoang70%m~mthongph:ittri~nva 30%
m~mphattri~nthanheh6idinhdu'ong(Anh3).
-44-
N6ngdQ
Gi6ng BAP Manvo Stfph:ittrin cuamm ngu
(mgll) trilng(%)
Mm thong ch6iDD Ch5iSS
PT (%) (%) (%)
24569 0 80 75 25 0
2,5 75 33,3 66,7 0
24799 0 70 71,4 28,6 0
2,5 82 22,2 77,8 0
24963 0 75 66,7 33,3 0
2,5 76 33,3 58,4 8,3
Phin NgmenCzi'u Luj..rJanPTS Khoah{X
. Khi co2.5mgllBAP , ti l~phattriSnch6idinhdu'ongcaohdn. Chi co 1
tru'CJnghQpphattriSnthanhch6isinhsanvdi ti l~8.3%.ra'tha'p.
Ngoaifa,ke'tquatrenclingchotha'yd3 loaikhacnhauthlti l~mftm
khongphattri~nclingkh,k nhau.d loai24963trongmoitru'CJngkhongco
nchthkht6till chico66,7%mftmkhongphattri~n,tha'phdnsovdi21oai
conl(,iivatrongmoitru'CJngco2,5mgllBAP till co 8,3%phattriSnthanh
ch6isinhsan,trongkhicaeloaikhactillkhongco.
Bg h(,inche'sr{tie'thQpcha'tphenoltli'm&uv~tca'ylamanhhu'dng
de'nphattri~ncuamgm,chungtoida:d~tca'ym&u(jvi tri ngu'QchiSuva
d~tnuoi(jnhi<$tdQ20°C.Ke'tquagillnh~nnhu'san:
Bangl. Nuoica'ycacdo(,inmangmftmngutrenphatboa(j20°C
Quan sat N6ng dQ cha't BHSTTV (mgll)
SvPT cua I M&uca'yvotrilng
1
13
2,5
12
5,0
14
10,0
12
~ ~ ~-------
):; ..
maIDngu MgmkhongPT (%) 23,1 8,3 0 25
trenphat ~hdiI)D-(~) r-38~5-T---25---1---14~3---r---o---
boa ~hdiSS-(o/~) r-38~5-T--66~7--1---85~i---r--7~--
Vitricua N1 - - 1 1
~ ~ ~-------
nhfi'ngch6i N2 - 1 2 1
sinhsan ~3 r--~---T i---1 3 r---2---
trenphat ~~ r--~--T 3---1 3 r---3---
~ ~ ~-------
boa N5 2 3 3 3
-45-
Phin NghienCd'u Lu?n an PTS. Khoa h<?C
K&tqualien chotha"yc;n6ngdqBAP 5 mg/l,tit ca caem~mdSu
phMtriSn,ti l~ch6isinhsantangkhoang86%,tfongkhi d6 c;n6ngde?
BAP 10mg/lthl ti l~n~ygiamcon75%.N6ngdqBAP caod milethich
hQp,das6cacvi tn m~mphMtriSnchofa ch6isinhsan,tfongkhi do c;
n6ngdQBAP thipchungchixuit hi~nc;vi tnm~ms64vasa5(Anh4).
-46-
PhanNghienCull
4
'."
\
!"j
~
a
c) --- -
-47-
Lu411;inPTS Khoa hoc
Anh 3.ch6idinhdu'ongphMtri~n
t11'mamngucuaphMboa
Anh4.ch6i sinhsanphMtri~n
111mamngucuaphM boa
Phli'nN ghj en Czfu Lu?n an PTS. Khoa h<;x:
BanR.J. Nuoi cf{ydinhngQnm~mngucuaphatboasan1thang.
Quan sat ! M6i tru'ongnuoi cf{y m~mII
M~ucffyvo trling(%)
M~ucffys6ngsan1tu§n(%)
M1
73,7
M2
64,7
M3
66,7
N1
50
0
81,8
0
41,7
0
Stf phM triSncua N2 0
0
+ 0
, . , J;: "-
cacv~tn maID tren N3
N4
++ +
phM boa + +++ ++.
N5 + +++ ++
+++:PhM triSnnhfi'ngk.h6iu nhochungquanhdinhngQn.
++ :Binh ngQnhlnhthanhvai 1an1§mmojo
+ :BinhngQnphongleunhu'ngsan2tu§nth1che't.
:BinhngQnkhongphMtriSnvasand6che't.0
M6i tru'ongM2 : Khoangdalu'Qngvavi lu'QngcuaMSb 1/2+0,34
mgllGA3+0,2mgll2iP.
Sankhi cffyvaomoitru'ongdaso'cacm~ucffykhongphMtriSnva
che'tnhanhchong.Ngo<;litrU'd moitru'angM2, ti 1t%n1~ucffys6ngsan1
tu~nla 81,8%caohdnsovoi moitru'angMl va M3, moi tru'ongM2 co
n6ngdQkhacaocuadu'ang60gll, agar6 gll va d pH 5,3va dIng d moi
tnfangn§ystfphMtriSncuacackh6iuchungquanhdinhngQnr1tdangkS,
d~cbit%td vi trimgmso'4va5.
-48-
Phin NghjenCIlu Lu;j.nanPT5. Khoa hpc
Bang3. Ti l~%cuam§ucf{yph:Hsinhcdquailva d~ctinhcua
Moi
tnfbng
Moi
cacb{iph~nmoi du'Qct~othanhsan8-10tugn.
M~uv~tc~y
Ti~nCll
I ( 5-6thang)
I
Caycon8-9T
.Than
.Chop1a
tnfbng I .Gifi'a 1a
Nl
Moi
.Cuo'ngIa
.La nguyen
Ti~nCll
( 5-6thang)
Caycon8-9T
.Than
.Chophi.
.Gifi'a13
tru'dngI .Cuo'ng 1a
N2 . La nguyen
M~uvo I M~u t~o
tillng (%) ch6i (%)
50 6
45
20
15,6
0
.
20 0
020
20 0
1050
50
18
20
11
20
20
0
10
17 12
-49-
D~ctlnheuaeh6i
I TCilOnhi~uti~nellnhu'ng
sandophattri~neh~m.
Nhi~uti~neu, eh~mpt.
Khongphattri€n
-nt-
-nt-
-nt-
TCilOnhi~uti~neu,
phattri€n blnhthu'bng.
Nhi~uti~neup.tri€nt6t
MQtsO'eh6iconphat
.f! ..,,!,! ,
trieD tCillv~tri vet eM.
Khong.
MQt sO'eh6i con p.tri€n.
Nhi~ueh6iconphat
tri€n t6tdph§ncu6ila.
Pl1anNghienCli'u Lu;jn ;inPTS. Khoa hc.x
dmoitru'()ngNl, nhfi'ngti~ncd(protocoffilS)du'qct~oto'ti~ncd ban
d~u.Tuy nhienti l~ chu'acaova sand6m§uv~tphattriSnch~m(A.nh5).
dmoitru'()ngN2nhi~uti~ncdmdidu'qct~othanh,chungphattriSn
blnhthu()ngvad cacvi tricuo'ngla,ch6pla clIngxua'thi~nch6iconC..\nh
§), (Anh7)vasanth()igian3thangcacch6iconnfiyrar~.
Ban~. AnhhuangcdalacdQngcdhQCdGns11t~okho'imo s~o.
M§u ca'y
Nguyenv~n
Ti l~t~okho'imo(%) Nh~nxet
0 -
(chi~usang)
-N~;yi~-;~~-r~ o ~ ~--------------
(trongto'i)
---C6-~~1~---T o ~ ~--------------
(chiGu.sang)
- -<:6-~~l~- -- T - - -- - --- - 4-0-- - --- - - - 1-8~~-';ftrhii~ ~d; kh6i -~-6d~~-
(trongto'i) dcaevGtdam.Caekho'imo
ptd~ngmobd(calfriable).
Khi dungklchthlchcdhQct~onhifngvGtthudngquanhgo'ccaycon
chu'axua'thi~nrSthldnhifngmfiuci{yd~tnuoitrongdi~uki<%nto'ic6ti 1<%
.
190kho'imocaokhoang40%,cackho'imon~yd~ngbd,vangnh~t(Anh 8),
.
(Anh9).
-50-
PilaRNgillen Cziu Lu#J;.in PTS. Khoahrc
Ban~. Anh hu'ongcuan6ngdQcha"tDHSTTV de'nslfgiatangsinhkh6i
cuakh6imo.
H6n hQpcha'tI TI. truckkill I Tl. sankhi I Tlnhtn;mgphattri~ncuamo
DHSTTV I ea'y(mg) I nuoi(mg)
MSBO 50 55 Mo kernpt.,mallnail den.
---1V[SBI---T 48 T---1-50---1JCh6i~6-~h~t(~;1~~~;p;~t~-
, , "
mall vang nail.
~ ~ ~---------------------------
MSB2 50 220 -nt-
---1V[SB3---T 52 T---5-00---1JCh6i~6-b~~~A-;i~g-;h;t-
- - -1V[SPO - - - T - - - - - 50- - - - - T - - - 200 -- -1 JCh6i - ~B - -~hit~- ~i~--; A~g-.
xanh.Xua"thit$n'nhfi'ngkhoi
di~mcuach6i.
-- - M SF 1- -- T - - -- -45- - - - - T - - - 603 -- -1 Phi~ -kh6i-~6-tlS'p-~d; -;di-
moi tnf<Jngtill ch~t.
--- MSF2--- T--- --48- T--- 1024---1JCh6i~6-b~~A~-;i~g-;;~h-.
(Anh10).
--- MSP3---T-- -- -51--- --T ---1540---1JCh6i~6-b~~~A~-;i~g-;h;t.-
Slf giatangsinhkho'icuakho'imo tily thuQcVaGhamItfQngcha"t
DHSTTVtrongmoitnf<Jngnuoi ca"y.HamluQngBAP eaos11gia tangsinh
kh6inhi~u(h6nhQpMSP3). Tuy nhien,dayla nhfi'ngd~ngmo ra"tbo, do
d6khaDangtai sinhkhi chuy~nsangmoitnf<Jngc6auxintha"p.
Phantiehphu'dngsaiehotha"yla slfkhckbit$te6y nghiaa mue99%
gifi'acaemoi tnf<Jng.
-51-
Ph/in NghH?n Cltu Luin in PTS, KhoahfJe
dkho'imohEsinhc6manxanhll)c(Anh l1.a),hlnhd,;mgcuakho'i
morAnch~chdn,d:;mgh<;it.(j mQtso'h<;itc6xu5thit?nnhfi'ngdinhhdinho
leu(Anh12).Trangkhid6,dkh6imachu'at,Hsinhthlmallvangnh<;it,bd,
khongc6 hlnh d<;ingro rt?t(Anh 11.b)
Quan satph~uthU'cc~tngang cua kh6i mo t,H sinh cho tha'yc6 nhiSu
h:;ttll)c l~pmanxanhva kill nhuQmvdi dungdichLugol chungan mall
xanhdu'dngd~m(Anh13).
Ban&.1.HamluQngdit?Pll)cto'cuakh5imo.
Vi cOdi~pll)cto'dkh5i motai sinh Dencu~ngdQQuanghQpva ham
lu'<;Jngproteincuakh5imotai sinhcao.
B~n~. Cu~ngdQQuanghQpcuakh5imo.
-52-
'Mu vt HamluQngdit?Pll)cto'(mgll)
Kh6imo -
Kh6irootaisinh 12,23
Mu vt CDQH (J.1102I g TL T I gi )
Kh5iroo -
Kh6irootaisinh 28,032
PhanNglllen CrJu LU?Il all PTS. Khoa hc.x
Bang-2.Ham1u'qngproteincuakh6imo.
Ban~. SlJ'taisinhcuakh6imo.
Khi SITdIJngANA trongmoitru'ongnuoi c3:yvdi nhfi'ngn6ngdQkhac
nbau,cacmoc3:yd€u cokhaDangtaisinhthanhcay.d moiWongco
n6ngdQANAcaoti1~taisinhcao,tuynhientrongmoitru'ongcochua3
mgllANA khiphattriSnthanhcayconchungcobQla varS cand6i,t6t
(Anh15)(A.nh16).
-53-
M§u vt Ham 1u'qngprotein (%)
i
Kh6imo
I
1,00
Kh6imotaisinh 1,88
Moi tru'ong N6ngdQ Ti 1 tai sinh Tlnh tr?ngphMtriSncuach6i
ANA (mgll) (%)
0,5 15 Ch6i viYanhuleD.
------------ ------------ -----------------------------
1,0 30 Ch6i nhu cao, co xu3:thit%nla.
------------ ------------ -----------------------------
1,5 50 Ch6i nhucao,
co 2 1axanhnh?t.
'
------------ ------------ -----------------------------
MS 3,0 >80 Cay conco 2-31axanht6t,
(j 1s6cayxu3:thit%nrS con.
------------ ------------ -----------------------------
5,0 >80 CayconcobQlaphMtriSn
kern,s6rSnhiu, ngn, trung
blnh3-4rS/cay.
PhliIllVghjeIlCd'u Lu?n in prs. Khoa hVC
. .
Anh 5. Slft~oti~nelldthancaycan.
I'
-- --
AOO6. Slfphattri€n cuach6icand ph~ncu6i13trongll10itru'bngN2.
-54-
Ph5nNghien Cliu LU~11an PTs. Khoa hQC
--
III
p
a
II"
ICI
II II
II
Anh 7. Stft~otiSncdtil'hi. .
,
II
.')..
II .. J',~j
III
Anh 8. Kh6i ruod g6cdiy con in-vitro
-55-
II
Phi/inNghienCtfu Lujn in PTs. Khoahrc
II .~
I .".
III
a
Q
... '),
"".1
!<
r '<.~I '
"""" ..' .~.'""""', ..
~
.
"
L
. .
Anh 9. PhButhucc~tngangthandvungt~okh5imo.
-- -
l
I
/'~
1:1 -
1:1 ..
IS
----
II r:::::= ~
~
Anh 10. Slfgiatangsinhkh6icuakh6imotrongmoitntC1ngMSP2.
-56-
PhinNgmenCliu LU?flanPT5. KhoahflC
cv
CL"
d
-.,~,
r.
...,.
...~
<
-.4'~
a
.- .
J
">
Anh 11.Kho'ill10taisinh(a)vakho'imochu'ataisinh(b).
I
-- .~.
g a
-- - ----
">
Anh 12. Dinhm~mxua'thi<$ntti'kho'imo.
-57-
Pilaf)]YghjenCdu LU?DanPTS, Kho3 h~JC
.'.
.."
:..."'-
, .">.
!w
'or
"
.
Anh 13. Phftuthucc~tngangkhO'iIDOconhuQIDdungdichLugol.
L ,
~ ~ -, -
Anh 14. Sddophauthucdit ngangvungt~oreo
-58-
Phl1nNghien Czfu Lu?nin PTS. Khoahf?C
~\....
I
I
I
I
,
~
Anh 15.S1J'taisinhcaycontiYkh5imo.
~ -
Anh 16.Stft~oredcayconin-vitro
-59-
rPhill Nghien Cd'u Lu?1J in PTS. Khoa h9C
PhfinHai : NGmtN c(fu NIlAN GIaNG VO TINH IN-VIVO
1.Md DAU
Nam 1959,Rotor[48],nghienCll'uvSsItsinhsancuagi6ngIann~y
chotha'ychungco2kieuch5im~m,1dnachla va 1dnhanhphatboa.Do
do,co2khananggayraslfearnU'ngtrenm~mba'tdinh:
- Ho~cbAngsItphattriencuadinhsinhtru'dngdnach13.
- Ho~cbAngsItphattriencuanhfi'ngmgmngudnhanhphatboa.
Vdi khanangthll'nha't,dethucd~ysItphMtriencuanhfi'ngmgmd
nachla, ngu'CJita dungky thu~tcAtbe dinhsinhtru'dngngQnCk)7thu~t
Topping)de phahuyhit%ntuqngu'uthe'ngQn(Zin1Illerva Pieper,1979)
[68], nhu'ngvdiphu'dngphapn~ytrungblnh1caychicho3-4ch5i,ngoai
faconanhhu'dngkhongt6tde'ncaymyVIdSlamna'mbt%nhva contrling
ta'ncongd vi tn ve'tthudng.
TrongphgnngymQtphudngphapnhangi6ngvo tmhbAngeachthoa
h6nhqpcha'tDHSTTV dekichthichsrfphattriencuanhfi'ngmgmngutren
phMboa.Phuncaedungdichdinhduong,theodoicaebie'nd5isinhly va
caechi lieu tangtru'dngphattriencuach&icon.ChQnmQtn&ngdQxu ly va
mQtmoi tru'CJngdinhduongthichhqpnha'tsaocho ht%so'nhangi6ngcao,
caycontangtru'dngvaphattrient6tmakhonganhhuangde'ncaymy.
-60-
Phin NghienClfu Lu#.nill PTs. Khoa hr,x:
2.VAT LIEU vA PmJONGPIlAF.
2.1.V~tli~u.
Cay IanH6 Dit%pda:tr6boadu'Qc15-20ngayva cay da:co phcHboa
chu'atr6 boa.Chi~ucao trungblnhcuaphM boa tll'40-50cm. Cac vi tn
m§mdIng du'qcdanhthu11;(tll'du'dig6cphatboaleDireD(vi tn m§m s6 1,
2, , vi tri g§nboandd§u tieD).
Nuoi tr6ngtrongdi~ukit%ncomaiche,cu'CfngdQanhsang2000-2500
lux,nhit%tdQbanngay28°C-30°C,bandem22°C-25°C,gmdQ80%.
2.2.Phuongphap.
2.2.1.Quansathlnhthaigiillphlu.
NhuQm2 mall,sonphenva IQCiod.
. Phftuthucc~tngangcuam§m(jvi tris61va2 (Hlnh4).
. Phftuthucc~tdqccuam§mdvi tri s63,4 vavi trim§mtrendIng san
khixuly cha'tDHSTTV(Hlnh5vaHlnh6).
Phftuthucc~tngangcqngphcHboangamtrongdungdichbicromat
kali.
Phftuthucc~tdqccqngphatboa(ArIh 17).
-61-
Phln NghienCli'll Luin ;inPTs. Khoa hc;x:
2.2.2.Dohamlu'<jngcha'iDHSTTV nQisinhcotrongphatboa.
CAtcaedO;;llleuaph:itboac6mangm~m,eachd~uteenva d~udu'di
m~tmangm~m1 emeuanhanhphatboa c6 boa chu'and va euanhanh
phMboacoboada:nd hoan toaD.
a.Ly triehvaphando~n
Stfly trichva phando~ncaechfftBHSTTV du'Qetrlnhbayt6mtAt
theosdd6san:(theoBili TrangVit$t,[11]).
Djchtriehmethanol80%
1 coc~n
Djehtantrongnude
Phaete
1
pH2,5
inchete
~ Fhanude
Phaaxit
1 pH7
sAcIcY
trichn-butanol
baaboanude.
"
coc~n
AUXIN
GIBERENLIN
AXIT APXIXI C
(d~ngttfdo)
XITOKININ
(D~ngt11do)
-62-
Ph!1nNghjenCd'u LU?flin PTS. Khoah9C
Chuthich:
S~ckythlfchi~nvdidungmoidi chuySn:
b.SAckY,xacdinhvi trivadohamluqng.
Thlfchi~nphuongphapsAcky trengiftyvdi giftyWhatmans6 1,d
nhi~tdQ30°C,dungmoi di chuySnla isopropanol:amoniae:nudc(10:1:1,
v/vlv)chocaenh6maux.,gib.vaAAB (Pilet,1961;Yokota,1980)[86,67].
Vi tri cuacaenh6mehfttDHSTTV trengiftysAckY va hamluqng
cuachungduqcxacdinhbdi cacsinhtr~cnghit%msan:
. D6ivdiauxin:
Ap d1}ngsinh tr~enghi~mkhlic e~tdit%ptieu Ilia Oryza sativaL.
(Nguy8nthiNgQcLang, 1970;Bili TrangVi~t,1989)[81,11].
Doslfgiatangchi~udaidi~plieusan24giCidStrongt6i.Hamluqng
aux.duqedotll'Rf 0,3-0,4(Senva Leopold,1954xemtrongBili Trang
Vi~t,1989)[11],tyIt%thu~nvdi srIsaibit%tehi~udaikhuccAtdit%plieuso
vdichuBn udcefttvaduQctmhbhngeachsovdimQtdungdichAlA tinh
khie't2mg/l.
. D6ivdigiberenlin:
Vi trigib.duQcxacdinhbhngcachnhlinggiftysAckYvaodungdich
pennanganatkali 5% (Harbone,1973xemtrongBili TrangVit%t,1989)
-63-
isopropanol 10
amomac 1
nudc 1
Phin No-menCzfub LU?D aDPTS. Khoa hrc
[11],gib.n~mtrongvlingtill nail trenn~ngia'ytrilng.Hamlu'<;5nggib. du'qc
do nhd sinh tr~cnghit%mcay m~mdu'achuQt(Hess, 1964xem trongBlii
TrangVit%t,1989)[11],ty lc$thu~nvoi srfsai bic$tchi~udai tfl}h~dit%pso
voi chu§:nnu'occa'tsan5 ngayxu ly, trongdi~ukic$nanhsang2000lux,
nhit%tdQ30°c, dQ§:mkhongkill 75%-80%,va du'QCtiuhbhngcachso sanh
voimQtdungdichGA3 tinhkhiSt20mg/l.
I . D6ivoixitokinin:
Ap d~ngsinhtr~cnghic$mla m~mdu'achuQtCucumissativusL.
(ThomasvaCSV., 1981,xemtrongBiii TrangVic$t,1989)[11].Hamlu'Qng
xito.t)7It%thu~nvoi srfsaibic$ttrQnglu'Qngtu'dicuala m~msovoi chu§:n
. .
nu'oea'tsan3 ngayxuly (gi6ngdi~ukic$n'cuagib.),vadu'Qetmhbhng
eachsosanhvoimQtdungdiehBAPtinhkhiSt1mgll.
. D6ivoiaxilapxixic:
A.p d~ngsinh tr~c nghic$mtren dic$p.th~uIlia Oryza sativa L.
(NguySnThi NgQcLang, 1970;Biii Trang Vic$t,1989)[81, 11],quahic$u
U'ngcansrI tangtru'dngcuadic$plieu. HamluQngAAB t)7lc$voi srIsaibit%t
chiSudai khuce~tdic$plieu sovdi ehu§:nnu'dcca'tva du'QCtinhbhng'cach
sosanhvoimQtdungdichAAB tinhkhiSt1mg/l..
2.2.3.XU'IychinDHSTIV trencaevitrfm4mngd.
CaccayIanH6Dic$pc6boadfftr5t11'5dSn20ngayvacaye6boa
chu'atr5.Chi~ucaotrungblnhcuaphatboatil'40dSn50cm.Vi tri euacae
mffmngudingdu'QedanhsO"nhu'trangnuoica'yin-vitro.Caev~yla baa
-64-
PhSnNghien Cd'u L#n anPTs.Khoahrc
quanhmgmdu'qctachbo,saild6xuly vdicach6nhqpcha'tDHSTTV nhu'
san:
. B1:0,1mgllxitokinin
. B2:0,1mgllxitokinin
- lanolin.
- lanolin+ 0,1mglla-PhenylHydroxylamin.
- lanolin.. B3:1,0mgllxitokinin
. B4:1,0mgllxitokinin - lanolin+ 1,0mglla-PhenylHydroxylamin.
. B5:10,0mgllxitokinin- lanolin.
.
. B6:10,0mg/lxitokinin -lanolin +10,0mg/la-PhenylHydroxylamin.
. B7:100,0mgllxitokinin-lanolin.
. B8:100,0mg/lxitokinin-lanolin +100,0mg/l a-Phenyl Hydroxylamin.
Caysailkhi xu ly du'qctu'dinu'dc2 19n/ngay.Tu'diphandinhdu'ong,
2Ifutltu~n,vaphundinhl~ thu5cTopsintrtngmb~nh2 l§n/thang.Cacdiy
du'<;JcMng (j di~uki~nchie'usang2000-2500lux,nhi~tdQtrungblnhban
ngay28°C-30°C,bandell 22°C-25°C,gmdQ80%.
2.2.4.Xii'lf dungd1chdiOOduoog.
.MPO:
. MPl:
. MP2:
. MP3:
. MP4:
cac moitru'C1ngdinhdu'ongdu'qcsud~ng:
D5i chUng(khongtu'didinhdu'ong).
KhoangdaIU'qngva vi lu'qngcuaMS (1962).
Khoangdalu'qngcuaMS +MgSO4.7H2O(250mgll)+
FeSO4.7H2O(30mgll)+ZnSO4.4H2O(75mgll).
Ure-Ian-kali(30-10-10)+FeSO4.7H2O(30mgll)+
ZnSO4.4H2O(8.6mgll).
Ure-Hin-kali(30-10-10).
-65-
Ph5n Nghien Cd'u Lu?n in PTS Khoa hc,x
2.2.5.Theodoicacbie'nd6i sinh15'trongquatrlnh hlnh thanhva
phattri~ncaycon.
Saukhi plumcacdungdichdinhduong.chungWi tiSn hanhdo cac
chitiensinh19cuacay:
a.Cu'(JngdQqnal1ghQp.
b.Cu'ongdQhoha'p.
BAngphuongphapapke'Warburg.doltl'<;1ngO2thaira trongmQtdon
vi thoigianldonvi tn;mglU<;1ngtu'oi.
c.Do hamluQngd?mt6ngs6trendly m~.
Thi nghi~mduQcb6 tn ng§unhien,m8imoi tru'ongdinh duongtudi
3cayvdi 3 19nl?p l?i. Ke'tquadu<;1cphantichbAngmayphantich d?mh1
dQngdTrungtamdichVl!phantichtill nghi~mthanhph6H6 Chi Minh.
Ke'tquaduqcphanitchb~ngphudngphapphantichphuongsai vdi bQ
chuangtOOhSTATGRAPHICS, phienban 7,0 (StatisticalGraphics
Corporation,1993).
d. Do hamluqngauxinnQi sinh (j cay eonbhngphuongphap sinh tr~c
nghiC;;m.
, 2.2.6.TheodOisrItangtr1iongvaphattri~ncuacay.
a. S11tangtru'dngva phMtriSncuacacmgmngusankhi xu 19cha't
DHSTTVbAngphuongphapdochi~udaicuach6i,ghinh~ncacbiSuhi~n
kiSuhlnhnhur~,than,la,mans~c.
b. S11tangtru'dngva phMtriSncuacaech6icon,gill nh~n861a,86r~,tmh
tr?ngphMtriSncuacaycon.
-66-
PhanNghien Cd'u Lu?n ill PT5. Khoa hfJe
3. KET OU~{VA NIlAN xET
Quansatph~uthuccAtngangm§mdvi tris61vavi tris62sailkhi
nhuQm2 mall (sonphen-1Qciod) cho tha'y:khongc6 phatthShi baaphil
vungsinhroo,belltrongcacb6mqchv~ncongidihqndph§ncorpuschu'a
n6ivdimqchd~nchinhdSnuoich6i(I-finh4).
RiCInhI
Nlm 1111)cllIIl!g ,
V(')JJO scfi ClI'cfJlj!, IlIl)b. ,
D;tlll s<.,5jcu'(jng lilt) trt' 'I hI') !ihe
B6 Jibe
130moc
Mwcl1 rl(~n
moo 4.Sdd6ph~uthucc~tngangm~mdvi tri 1va2.
Quansatlat c~tdQcvi tri m~ms6 3 va s6 4 sailkill xU'ly cha't
DHSTTV chotha'ychungphattriSnchofa nhffngch6idinhdu'ong, vdi
nhffngv~ylabaaquanhdinhm§m(Hlnh5)vadlatc~tdQcvi trim~ms65
chod~nvi trim~mg~nboandd§ulienla srIphattriSncuanhffngch6isinh
san(ffinh6).
-67-
Pl1lnNghienCUll Lu?nin PTS.Khoah9C
moo 5.Lat cAtdQcvi trim~ms53 va 4 trencQngphathoa
(Ch5idinhdu'ong)
moo 6.Lat cAtdQcvi trim~ms55 va 6 trencQngphathoa
(Ch5isinhsan)
-68-
PhanNghjen Czi'u Luan anPTS. Khoa h<?C
HamIu'Qngcuacaecha'tDHSTTV co trongnhanhphMboadu'Qctie'n
hanhphantichb~ngphu'ongphapsinhtr~cnghit%m(xerntrongmt;lc2.2.2),
ke'tquadu'QCgill nh~nnhu'sau:
BangJ1. St1bie'nddihamlu'Qngcaecha'tDHSTTV trencaevi trim~m
cuanhanhphcitboa danohoantoan.
Vi trim~m
1
Ham lu'Qn cha't DHSTTV (m!l)
Aux. Gib. Xita. AAB
0,13 2,07 0,51 7,9
---1~1--~- 1~93 T o~53------5~Ol---
==~~========HF===f====~~===~==~~===
---4:;7 3~89---~T O~20------1~93---
------------
2------------
3------------
4------------
5
B,A> d~ 2leu 0 .
Sl[ BIIfN DdI HAM LuqNG CAC CHArT DHSTTV TREN
CAC VI TRi MAM CUA PH AT HOA DA Nd
.-. 8
~
E 7
....
E-< 6E-<
rI:J
::z:: 5Q
E-<
'< 4:I:
U 3~
~ 2
J
~ 1
~
:I: 0
151Aux.
IIIDGib.
IIIXito.
51AAB
1 2 3
VI TRt M..{M
4 5
-69-
Pha'nNghienCzfu Luj.n:inPT5. Khoahc;x:
Ban~. Slfbie'nd6ihamlu'Qngcaecha'tBHSTTV trencaevi trimfim
euanhanhphMboaehu'alid.
Bi~u <163.
sT.fBIEN BOI HAM UJqNG CHAT BHSTTV TRtN CAC VI TRf
MAM CUA PHAT HOA CHVANd
ElAux.
11mGib..Xito.
IIIIIAAB
2 3
VITRfMAM
4 5
-70-
Vi tri mftm Ham lu'Qngeha't BHSTTV ( mg/l)
Aux. Gib. Xito. AAB
1 1,30 2,11 0,42 5,21
----------- ----------- ------------ ------------ -------------
2 2,35 2,21 0,39 4,32
----------- ----------- ------------ ------------ -------------
3 2,52 1,57 0,33 2,11
----------- ----------- ------------ ------------ -------------
4 1,14 494 0,81 3,26,
----------- ----------- ------------ ------------ -------------
5 6,58 8,05 1,39 1,08
9.00
8.00
7.00
6.00
, 5.00
u
Z 4.00
g
3.00
2.00
1.00
0.00
1
Ph5nNghjen Czi'u Lu?n;inPTS Khoahex:
HamluQngaux., gib., xito.,tangd§n tU'vi tri sf)1d€n g§nvi tri boa
ndd~utieD,trongkhi dohamluQngAAB giaml~n.
So sanhhamluQngcuacaech:1ttrend nhanhphatboacoboachua
ndvad11ha11hphatboacoboada:ndhoanto[111choth:1yluQngaux.,gib.,
xito.dnhanhcoboachuandcaohdn.Nhu'ngVId~xacdinhrohdnvScae
d~ctinhcuaboava d~thudngthll'cboamQtthaigian,d6ngthai,ke'thQp
voislfquailsatphatboa,lienchungtOida:chQnphatboacoboandhoan
tOaDd~tie'nhanhthinghi~mnhangi6ngvatint.
Bang13.AnhhuangcuandngdQch:1tDHSTTV trencaem§mngu
cuaphatboasan2 tugn.
H6nhQp
."1'Xu Y
Bl chdi phattriSn,xanh,chiSudaiva du'ong
~~~~F~t~~~~~~~~~~~~~~~~:~~~~~;~:~f~i~~~~~~~~~
Q!~tc§y-§-Yi !~t~-~~§~- --- - - - - - - - - - - - -- - --
-nt-----------------------------------------
chdi phattri~n,xanhtf)t,t~ophM boa full'
'" , . "5 a6£~P_~_~l~__Y__-~-----------------------
chdi phMtri~n,xanhtf)t,gia tangchiSu
daiva duongkinh dangk~,tqOphatboa
~ i r~!t;~~~~~~~kfuh~Y~~~~~
bSmatnhaleDnhiSudinhmgmxanhdam.
~ l r ~ =~t: ~---
Tlnhtrqngphattri~ncuachdi
r---B4--
1
. 2-------
[~~~~~ ~~~~~~~~~~~ ~~
I B6 I 2
B7
B8
-71-
Pl1in Nglllen Czfu Lu;jnallPTs. Khoah9C
Sailkhi xu ly caeh6nhQpeha'tBHSTTV khaenhalltrencaevi tri
mall nguehotha'ycaem~mngud~ud.mll'ng,m~mnhalen,dmQts6vi
trimamxua'thi~Ilphatboathuea'p.C5 n6ngdQcae(h6nhQpB7 va B8),
thaigianmamearnU'ngnhanhnha'tla 1ngay,xua'thi<%nnhi~udinhmamtti'
mQtdinhmambandati,du'ongkinhmamtangnhanhnhu'ngnhfi'ngmam
naykhongphattriln tie'pthee d6. C5 h6nhQpB6, ch6i phattriln t6t,gia
tangehieudaivadu'ongkinh.Do d6,h6nhQpB6 du'QehQndl theodOi
caetmnghi~mtie'pthee.
Ban&.14:.Cu'ongdQquanghQpeuacaevi tri m~m
sankhi tu'oidinhdu'ong1tHan.
-72-
Vi trimam Cu'ongdQ quanghQp J.Ll02/gr/g.
MP1 MP2 MP3 MP4 D6ichUng
1 31,583 31,023 31,072 30,487 20,120
2 32,974 33,011 32,983 30,987 21,080
3 33,294 34,215 33,021 31,206 21,720
4 40,605 42,120 39,601 32,028 21,040
5 66,170 68,160 65,425 53,360 23,720
Phin NulllenClfu/:J Lu?n;inPTS. Khoahrc
B,R d~ 4leu 0 .
Cu'&ngde;quanghqpcoocaevi trl m~msaukhi tu'ifIdddinhdu'ong1
tulin
70
01
012
Eil3
114
m5
60
.! 50
CII-=.s!'
§ ~ 40cr...
co-'"~ 0 30
[
:
.
<
CII::S ..
~-;:;"20::u
10 .
0
MPl MP2 MP3 MP4 001
CHTJNG
Vi tri~m
BaO215. Cu'~ngdQhoha'pcua~acvi trim~m
sankill tu'didinhdu'C1ng1tnftn,
-73-
Vi tri mftm Cu'ng dQ he>ha'p J1l01lgr/g
MPI MP2 MP3 ' MP4 D6ichung
1 15,298 14,936 14,011 13,211 10,720
2 26,010 25,558 22,538 21,098 15,600
3 29,323 29,325 27,120 27,180 16,121
4 41,010 40,987 42,128 39,721 20,021
5 41,182 42,648 42,150 40,015 20,800
Pha'nNghjenCUll Luin anPTs. Khoa h9C
Biiu d65.
Cu'ongdQh6hti'pcoocacvi trfm~msaukill tu'didddinhdu'Ong1twin
45
40
35Q;
~~30
~ ... 25.c~
<0- N
"= 0 20!i!i-
tg~ 15 Iu 10
5f
0
m1
m2
El3
m4
m5
MPI MP2 MP3 MP4 001
CHUNG
Vi trfrmim
Sankhi thll'nghi~mphunCaedungdich dint du'(jng;Chungtoi nh~n
tha'yd moi tru'e;ngMPI va MP2 till CE>QHva CE>HHgia tangcaohdn so
vdicaemoitru'e;ngkhacvasItgiatangdIngtheothutlftti'vi trim~n1s51
chode'nvi trim~mg~nboandd~utieD,lienchungtoi chQn2 moitru'e;ng
trend€ tie'ntanhtheod5icaechitieucuacay.
Ban~. Cu'e;ngdQQuangh<;Jpvacu'angdQhohffpcuacaevi trlm~m
sankhiphundungdichdint du'(jng2 tu~n.
-74-
Vi tri mm MP2 (,.u02/gr/g) MP3 (J.!l02/gr/g)
CE>QH CE>HH CE>QH CE>HH
1 35,010 15,010 33,250 -___t4.,9l__-------------- ------------ ------------ ----------
2 ___2}__- 29,601 52,817 22,692------------- ------------ ---------- -------------
3 98,150 38,260 81,110 --_J_4.,§)-Q---------------- ------------ ------------ ----------
4 --)_,}}2__- 48,180 10,110 49,025------------- ------------ ---------- -------------
5 15,101 50,218 14,103 49,970
Ph/inNgmenCzi'u Lu:jnin FrS. Khoah9C
B'R d~ 6leu 0 .
CliongdQquanghlipvacliongdQM h:fpcnacacvi tri m~msankhitlioidd2
t~n
160
140
ee 120
.§ ~ 100
... ...
~ ~
80.= ,.
c c::>
:~;:60"0-'0
~
~
40
20
lID MP2 CE>QH
mMP2 CE>HH
E! MP3 CBQH
mJMP3 CBHH
0
2 3 4 5
'1tri~m
Cu'C1ngdQquanghQpVa cu'C1ngdQho ha'psail khi phun dungdich
dinhdu'ong2 tu~ndSugia tangtU'vito s6 1d~nvi to g~nboand d~ulien.
Tuynhien,ddungdichdinhdu'ongMP2 caohonvadvi to m~ms64 va56
5CDQHvaCDHHtangcaottfongU'ngvdi srIxua'thi<$ncuacaech6idinh
du'ongvach6isinhsandnhiIngvi to n~y.
Ban~17.HamIu'Qngd~mtangs6,
-75-
Moi tru'C1ngtu'di HamIu'Qngdm tangs6(%)
sail2 tun tu'didd.dinhdu'ong
MP1 1,634
----------------- ------------------------------
MP2 2,028
----------------- ------------------------------
MP3 1,756
----------------- ------------------------------
MP4 1,608
Pha'nNgllien Cd'u LU:fll allPT5. Khoa h9C
Bi~u d&7.
HAM LUQNG D~M TONG s6 TREN cA Y IVIJ::SA U KHI
TUdI DD.DINHm.1CJNG2TUAN
'<0 2.5'"
'"'
~
f-<
~ 1.5
"*-
a«f<
'"'- 1Z
g
~ 0.5
~
~
2
0
WP1 MP2
M6I TRUONG DINH DUONG
II MPI
II MP2
tl1MP3
mMP4
M'3 ttF4
.
PhantichhamIu'Qngd(;lmt6ngs6trencaym~chothffy,dmoitntong
dinhdu'ongMP2 caohon caemoi tru'ongdinhdu'ongkhac.Phan tich
phu'ongsaiehothffysrfkhacbit%tv~hamIu'Qngd(;lmt6ngs6la e69 nghlaa
mue99%.
Ban~. SrfphMtri~ncuacaem~mngiisan2thangxii'19chfftDHSTTV.
Vi tam~m
1------------
2------------
3
ch6itangdai(em)
0-------------------
0-------------------
3,5
--------------------------------
4 7,0
--------------------------------
5 12,0
Tlnhtr(;lngphMtJ.i~ncuach6i
l1~~_~§~g_e~~!J~~~-----------
-nt---------------------------------
Ch<3ipt t6t,xuffthit%n2 la, d6ng
~~!~tI!<!~gJ~_il}l!-~~~~i~_t~.!lk -- --
Ch<3ipt t6t, c6 2-3 la, du'dig6c
~h~_~~~!!ti§~-~:~~~~ _C_~I!.'-- - - --
Xua'thit%nphMboathucffp,c65
v~y13mangm~m,vi tri m~m1
nhocaoxuffthit%nla.
-76-
PhanNghjen Czfu Lu/jn anPTS. ](hoah9C
Sau2 thangxU'1yh6nhqpchit BHSTTV B6 va phundungdiehdinh
du'ongMP2, ke'tquaghinh~nnhu'sau(Anh 18):
- d vi tri m§m 1va 2 khongphattri~n.
- d vi trim§m3va4xuit hi~neh6idinhdu'ong.
- dvitrim§ms65xua'thi~neh6isinhsan(ph:itboathll'ca'p).
BanfU2.Hamlu'qngauxinnQisinha eh6icon.
Phantichham1uQngauxina eh6icontreneQngph:itboachotha'y
khitu'didungdiehdinhdu'ongMP2, hamluQngaux.nQisinhva s61u'Qng
eh6iconxria'thi~n1aeaohdnadungdichdinhduongMP3.
Ban~20. Stftangtru'ongvaph:ittri~ncuacaech6iconsan4 thang.
3------.
4------
5
s6 luQng eh6i
, c6 hi
MP2 MP3 MP2 MP3
3 2 0 0 +----------------------
3 3 2 1
:l~-:;:i~~~-- 3-- - --34-- -:::;~:T~~~-~:::~~~~~t.t!~-
boafull'ca'p I th~tachkhoi cay m~
d~tr6ng.
S6 luQugr~I Tlnh tr~ngph:it triSnVi trl
i{mam .. "cnacaycon
-77-
M§u vt Ham1u'Qngauxin(mgll)
Caycon(dinhduongMP2) 4,20
Caycon(dinhdu'ongMP3) 3,65
Phin Nghj{~nCziu Lu?D :inPT5. Khoa hrx:
Caccayconkhicokhoang3-41ava3-4rSconcothi tachkhoi cay
m~di tr6ngvaonhU'ngch~nrieng(Anh19,Anh20).San12-14thangcay
setr6boa.Tny nhien,nhU'ngphMboan~ynhovang~nVI ra boal~nd~n
(A.nh21).
III
..
bJ
III
'.. ..
.. - I
II
II
I!JII-
D
\ "'"".
,#'
I!'
,.
( ill
a
--
~ , ..
Anh 17.LlJc l~ptrongtebaocuaphMboa.
-78-
Pha'f1Nghien Cziu Lu?n anPTS. Khoa hc,x
AOO18.Ch6idinhdu'ongvach6isinhsanxngthi~n
sankill xuly vdihanh<;1pB6.
III
AOO19. Cay conphi:itriSntll'caem~mngucuaphi:itboa.
-79-
Phin NghienCziu Lu;j.nill PTS Khoah9'
lnh 20. CayconphMtri~ncodgydiihivar~.
II!
,.
Anh 21. Cayfahoa19ndgu.
-80-
