The biological characteristics of interactions between “episyrphus balteatus de geer” insectivore hoverfly on ceratovacuna lanigera (zehntner) in Tho Xuan district, Thanh Hoa province

Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 109 THE BIOLOGICAL CHARACTERISTICS OF INTERACTIONS BETWEEN “EPISYRPHUS BALTEATUS DE GEER” INSECTIVORE HOVERFLY ON CERATOVACUNA LANIGERA (ZEHNTNER) IN THO XUAN DISTRICT, THANH HOA PROVINCE Le Van Ninh1 Received: 2 August 2016 / Accepted: 10 October 2017 / Published: November 2017 ©Hong Duc University (HDU) and Hong Duc University Journal of Science Abstract: The growth process of Episyrphus balteatus passes 4 stages of development: egg, larva (maggot), pupa and adult. The average life cycle time of the fly at 27.8 0C is 18.7 days, at 25.3 0C is 20.1 days. The average life time of the fly at 27.8 0C is 25.9 days, at 25.3 0C is 28.6 days. The fly of E. balteatus lays eggs within 9 days of insemination with an average of 39.5  2.11 eggs/adult fly. On the sixth day, the maximum numbers of eggs are 5,6 eggs/adult/day. At mean temperature condition of 25.3 0C, mean moisture of 82.5%, an 1- instar maggot eats an average of 17.2 ± 0.37 aphids, a 2-instar maggot eats an average of 69.7 ± 0.62 aphids, a 3-instar maggot eats an average of 196.2 ± 2.15 aphids. At a temperature of 27.80C, and moisture level of 77.6%, the maggot eats fewer aphids. An 1- instar maggot eats an average of 15.9 ± 0.44 aphids, a 2-instar maggot eats an average of 57.5 ± 0.51 aphids, a 3-instar maggot eats an average of 188.7 ± 1.84 aphids. Keywords: Episyrphus balteatus, hover fly, sugarcane wooly aphid, predation rate. 1. Introduction 1.1. Background During the development and growth process, sugar-cane is readily harmed by pests that cause reduction in productivity and quality of the raw sugar-cane. Among these pests, sugarcane wooly aphid (Ceratovacuna lanigera (Zehntner)) is the main pest having a rapid life cycle and high rate of increase of populations that can lead to major pest outbreaks. However, in the natural environment, Ceratovacuna lanigera (Zehntner) is restrained by natural enermies, especially the ladybird beetle (Coccinellidae), and the aphid-eating hoverfly (Syrphidae), parasitic wasp (Encytidae). The aphid-eating hoverfly (Episyrphus balteatus de Geer) plays the most important role in restraining numbers of C. laniger. When the density of the larva population of aphid-eating hoverfly is high, it is able to restrain successfully the C. Lanigera population. This study examines the intereaction between this hoverfly and the sugarcane wooly aphid in Tho Xuan district, Thanh Hoa province in Vietnam. Le Van Ninh Faculty of Agriculture, Forestry and Fishery Department, Hong Duc University Email: Levanninh99@gmail.com () Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 110 1.2. Monitoring methods in the field Monitoring procedure was conducted in accordance with QCVN 01-38:2010, monitoring protocols. The varieties of sugar cane in the study field were ROC22, ROC10, VĐ94 -128, MY55 -14. The study sites were in lowland and highland locations. The planting spacing ranged from 1 m to 1.25ms. We monitored each crop for seven days each session. We assessed two plants every 5ms for presence of aphid-eating hoverfly and sugarcane woolly aphids. We observed the top and lower surface of leaves and the other parts of each plant, counting them and then catching a sub-sample to bring to the laboratory for verification. 2. Research method in the laboratory 2.1. Research method for the biological and ecological characteristics of E. balteatus hoverfly in laboratory’s conditions Mature E. balteatus hoverflies were caught in the field then placed into an insect net cage where VDD94-128 variety and sugarcane woolly aphids were established to simulate a natural environment. We monitored daily for fertilized E. balteatus eggs then leaves with eggs were placed in a worm bin to assess the duration until hatching. Moist filter paper was also put inside the bin and the the petioles were wrapped with macerated cotton to keep the leaves fresh. The leaves were changed every 2 days. After recording the egg-openning period, we monitored the feeding stage of the 1st- stage larva (maggot) in the worm bin (n=30). In each “worm” bin there were moist filter papers, sugar cane leaves and sugar cane woolly aphids. Afterthe pupa emerged as adults, we selected breeding pairs of flies of the same emerging-dated and placed them into a net cage where the host plants and aphids were available. 2.2. Method of identifying the predacity of E. balterus flies under laboratory conditions Daily at 0800 hrs, we added 50 aphids (1st and 2nd instars) in each of 30 worm bins, counting regularly the remaining aphids on leaves. We monitored the predation rate until the larva transformed into a pupa. 3. Findings and discussion 3.1. Morphological characteristics and dimensions of the development phases of Episyrphus balteatus insectivore hoverfly on Ceratovacuna lanigera In each different development period, E.balteatus eggs varied in shape, dimension, and color. To identify exactly every development phase of this fly, 10 individuals of each phase were fed at an average temperature: 26.5 0C and average huminity of 83.5%, which achieved the results as follows: Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 111 Eggs: the eggs were tubular-shaped, new-laid eggs were white color, then turned into milkiness color when eggs were close to hatching. Dimensions of eggs: 1.57 ± 0.08 mm in length, 0.66 ± 0.10 mm in width. Larva: referred to as maggot type with 3 instars. Pure white color when emerging from eggs 3.5-4 mm in length and 1-1.5 mm in width. Full-grown maggots were 10.5 ± 0.45 mm long and 2.45 ± 0.16 mm wide, double-end oblong and flat-body shape, mouthpart pull-back into the front end. Pupa: at the begining of puparation time, pupae were the same color as larva. When nearly emerging as adult, the color turned darker. Pupae have large head and a smaller tail with a mean length of 7.23 ± 0.35 mm and mean width of 2.91 ± 0.25 mm. Adult: the female has a larger abdomen than the male, with an egg sticking tray. The male has a flat abdomen, and thinner and longer body than the female. The female mean body length was 8.7 ± 0.58 mm, mean width 2.44 ± 0.22 mm, with a mean wing span of 17.44 ± 0.74 mm. The mean body length of a male was 9.43 ± 0.5 mm, the mean width was 2.49 ± 0.23 mm, and mean wing span 17.81 ± 0.73 mm. The compound eyes are red brown color and occupy most of the size of the head. Figure 1. Photo of development stages of E. balteatus (de Geer) fly Aldult stage Egg stage Pupa stage Larva stage (Photo: Taken in June/2013) 3.2. Biological particularity of aphid-eating E. balteatus hoverfly Characteristics of development stages Egg stage: eggs of E. balteatus hoverfly are laid scatteredly on the surface of sugarcane leaves. Eggs are laid near, or on, the aphids. Development stage of egg was from 1 to 3 days. Larva (Maggot) stage: maggots began to consume prey 3hrs after emerging. They showed preference for eating the 2-instar aphids than the 1-instar aphids. The larva stage has 3 instars. Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 112 Pupa stage: pupa’s color is like that of the larva at the begining, with 2 black bands on its back. When nearly emerging to be an adult, the pupa’s color tends to be darker, and turns to light brown when the adult body can be seen through the cocoon. Adult stage: adult emerge in the morning and is the most active in the early morning or in the late afternoon. When coming out of the cocoon, the adult’s body is weak. After a few hours, the body is harder and the body’s color turns darker and darker but cannot fly yet. The adults prefers light so it often moves toward the bin’s cover. Table 1. The development time of stages of E. balteatus fly which is fed C. Lanigera in the laboratory Development Stages Development time of stages of E. balteatus fly (day(s)) 1st Feeding Period 2nd Feeding Period Max. Min. Average Max. Min. TB ± Se Egg 3 1 2.35 ± 0.203 3 1 2.18 ± 0.205 Larva 8 4,5 7.4 ± 0.266 8 4 6.98 ± 0.345 Pupa 9 7 8.45 ± 0.24 8 6 7.87 ±0.147 Adult 10 6 8.55 ± 0.378 8 5 7.28 ± 0.248 Before laying 3 1,5 1.87 ± 0.202 3 1 1.75 ± 0.207 Life cycle 24 16 20.07 ± 0.543 22 12 18.7 ± 0.617 Life 34 20 28.62 ± 0.759 29 17 25.93 ± 0.75 Average Temp. (oC) 25.3 ± 0.75 27.8 ± 0.78 Average Humidity (%) 82.5 ± 1.83 77.6 ± 1.77 (Note: n = 30 individuals) 3.3. Fecundity and frequency of birth of the flies of E balteatus The fecundity of E. balteatus and its frequency of laying batches of eggs depend on the environmental temperature and moisture conditions. We raised 10 couples of the adults. The daily quantity of eggs and total of hatched eggs are shown in table 2. Table 2. Fecundity of E. balteatus raised by feeding on the aphis of C. lanigera under different temperature condition in the laboratory. Date of egg dropping Quantity of eggs (number/mature/day) Temp. (oC) Moisture (%) Minimum Maximum Average 1 1 3 2,4± 0,50 26,7 81 2 2 4 3,2 ± 0,56 28,2 83 Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 113 3 3 5 3,8 ± 0,56 27,5 79 4 3 6 4,2 ± 0,66 29,2 74 5 4 7 4,6 ± 0,69 28,1 68 6 5 8 5,6 ± 0,69 29,3 85 7 5 7 5,4 ± 0,67 29,1 83 8 3 5 3,5 ± 0,51 26,8 79 9 1 2 1,28 ± 0,30 27,3 80 Egg dropping ability 27 43 39,4 ± 2,11 27,6 81,2 (Note: n = 10) The flies of E. balteatus drop eggs within 9 days, the frequency of egg dropping gradually increased and reached the maximum quantity on the sixth or seventh day (5.3 - 5.6 number/mature/day), then decreased to the minimum of 1.28  0.38 number/adult/day on the next day. At the temperature and moisture conditions of the laboratory, their average egg dropping ability is 39.5  2.11 number/adult (Table 3.3). 3.4. The rate of hatching of E. balteatus in the laboratory The rate of hatched eggs is an important biological criterion to identify the quantity of flies of the next litter that are likely to develop in the field. If the rate of hatched eggs is low, even though the fecundity of the adult is high, if the quantity of prey in the field is high, then the quantity of flies is not sufficient to manage the pest population. Therefore, we examined the rate of egg production of adults raised in the laboratory. The results are shown in table 3. Table 3. The rate of hatched eggs of the fly of E. balteatus in the laboratory Checking date Total of check eggs (nos) Total of hatched eggs Average temperature (oC) Average moisture (%) Quantity (nos) Rate (%) 10/5 - 12/5 38 34 89,5 26,4 87,4 15/5 - 17/5 32 26 81,2 25,8 77,9 19/5 - 21/5 35 28 80,0 25,6 83,4 23/5 - 25/5 33 30 90,9 26,7 87,8 28/5 - 30/6 40 34 85,0 28,6 81,4 2/6 - 4/6 34 24 70,6 29,7 75,9 At different temperature and moisture conditions, the rate of successfully hatched eggs is different. At the average temperature condition of 27.10C and humidity of 87.8%, the rate of Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 114 hatched eggs reached 90.9%. At 29.70C and humidity of 75.9%, the rate of hatched eggs was 70.6% (Table 3.4). Therefore, temperature and moisture are two factors which affect the rate of hatched eggs in the labortory. 3.5. Identifying the ability of eating the aphis (C. lanigera) of the flies of E. balteatus The flies of E. balteatus play an important role in controlling the quantity of the aphis of C. lanigera. In order to examine the consumption by E. Balteatus of the aphids, we assessed the level of consumptions of 1,2 -instar aphids. The consumption by E. balteatus of both instar stages is high (Table 4). Table 4. Predacity of the E. balteatus fly on aphids (C. lanigera) Age Predacity of maggots through the raising stages Stage 1 Stage 2 Minimum Maximum Average Minimum Maximum Average Maggot at 1-instar 15 19 17.2 ± 0.37 13 18 15.9 ± 0.44 Maggot at 2-instar 62 73 69.7 ± 0.62 51 67 57.5 ± 0.51 Maggot at 3-instar 175 207 196.2±2.15 168 192 188.7±1.84 Average temperature (0C) 25.0 ±0.75 27.8±0.78 Moisture (%) 82.5±1.83 77.6±1.77 4. Conclusions and recommendations 4.1. Conclusion The growth process of E. balteatus passes through 4 stages of development: egg, larva (maggot), pupa and adult. The average life cycle time of the fly at 27.8 0C is 18.7 days, at 25.3 0C it is 20.07 days. The average life time of the fly at 27.8 0C is 25.93 days, at 25.3 0C it is 28.62 days. The flies of E. balteatus lay eggs within 9 days with an average of 39.5  2.11 eggs/adult fly. The maximum numbers of eggs are laid on the sixth day (5.6 eggs/adult/day). At the average temperature condition of 25.3 0C, and average moisture of 82.5%, an 1- instar maggot eats an average of 17.2 ± 0.37 aphids, a 2-instar maggot eats an average of 69.7 ± 0.62 aphids, a 3-instar maggot eats an average of 196.2 ± 2.15 aphids. At the average temperature condition of 27.80C, and average moisture of 77.6%, the maggot eats less aphids. Hong Duc University Journal of Science, E.4, Vol.9, P (109 - 115), 2017 115 An 1-instar maggot eats an average of 15,9 ± 0,44 aphids, a 2-instar maggot eats an average of 57.5 ± 0.51aphids, a 3-instar maggot eats an average of 188.7 ± 1.84 aphids. 4.2. Recommendation The findings from this study provide a reference document for students whose major is crop protection at universities and colleges. References [1] Ministry of Agriculture & Rural Development (2001), Standard for plant protection (Book 1), Standard of Agriculture of Vietnam, Ha Noi. [2] Ha Quang Hung, Nguyen Thi Hong (2005), Episyrphus balteatus de Geer - family Syrphidae, density variation of Episyrphus balteatus de Geer and aphis on cucumber tree of winter-autumn crop in 2004 in Gia Lam, Hanoi, Science report at the fifth national insectology Seminar - Hanoi, 11-12/04/2005. [3] Nguyen Viet Tung (1990), Some comments on natural enemies of Aphis medicaginis caused damage to the Hong river delta, The first insectology seminar in Vietnam, Page 76. [4] Krishan, C. Sharma and Orn P. Bhalla (1990), Syrphids in the management of aphid pests of vegetable crops, India, 3rd Internation Conference on plant protection in the tropics - Malaysia, p 260. [5] Mutin, V.A (2005), The Japan sea region as center of Syrphid endemism and dispersal center of arboreal faurna, 3rd International Symposium of Syrphidae, Leiden 2 - 5 September 2005.