Luận văn Khảo sát các yếu tố ảnh hưởng đến chất lượng sữa bắp tiệt trùng

Cân các môi truờng nuôi cấy và hòa tan trong nước cất với hàm lượng xác định trong bình cầu (hoặc tam giác). Dùng bông gòn bịt kín miệng cầu. Đun nóng môi trường để hòa tan hoàn toàn các thành phần môi truờng trong nước. Pha loãng dung dịch nước muối 0,85%. Dùng ống đong, đong 90 ml nước muối đã pha loãng cho vào bình cầu. Bịt kín miệng bình cầu bằng bông gòn. Rữa sạch các đĩa petri, ống nghiệm, pipette. Dùng giấy bịt kín (chuẩn bị thanh trùng) Thanh trùng các môi trường, dụng cụ thủy tinh, bình chứa mẫu pha loãng,. 1.5.2 Chuẩn bị mẫu Tiến hành hút 1ml mẫu từ nguyên liệu (sản phẩm sữa) cho vào ống nghiệm đựng 9 ml nước muối 0,85% (pha loãng mẫu theo tỉ lệ 1/10).

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---------------------------- 54 - 64 *0.174167 0.13608 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xxi ANOVA Table for L by Mau Thi Nghiem.TP Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 1.00977 6 0.168295 1.83 0.1065 Within groups 5.97076 65 0.0918579 ----------------------------------------------------------------------------- Total (Corr.) 6.98053 71 xxii Multiple Range Tests for L by Mau Thi Nghiem.TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 62-65/90 9 78.4611 X 62-65/60 9 78.4811 XX 52-55/90 9 78.6089 XXX 52-55/60 9 78.6156 XXX 62-65/30 9 78.7344 XXX 52-55/30 9 78.75 XX DC 18 78.7683 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 0.134444 0.285339 52-55/30 - 52-55/90 0.141111 0.285339 52-55/30 - 62-65/30 0.0155556 0.285339 52-55/30 - 62-65/60 0.268889 0.285339 52-55/30 - 62-65/90 *0.288889 0.285339 52-55/30 - DC -0.0183333 0.247111 52-55/60 - 52-55/90 0.00666667 0.285339 52-55/60 - 62-65/30 -0.118889 0.285339 52-55/60 - 62-65/60 0.134444 0.285339 52-55/60 - 62-65/90 0.154444 0.285339 52-55/60 - DC -0.152778 0.247111 52-55/90 - 62-65/30 -0.125556 0.285339 52-55/90 - 62-65/60 0.127778 0.285339 52-55/90 - 62-65/90 0.147778 0.285339 52-55/90 - DC -0.159444 0.247111 62-65/30 - 62-65/60 0.253333 0.285339 62-65/30 - 62-65/90 0.273333 0.285339 62-65/30 - DC -0.0338889 0.247111 62-65/60 - 62-65/90 0.02 0.285339 62-65/60 - DC *-0.287222 0.247111 62-65/90 - DC *-0.307222 0.247111 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Analysis of Variance for a - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Thoi gian TP 0.176506 3 0.0588352 1.55 0.2094 B:Nhiet Do TP.so 0.0329389 1 0.0329389 0.87 0.3547 RESIDUAL 2.54131 67 0.0379299 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 2.75075 71 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. xxiii Multiple Range Tests for a by Thoi gian TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Thoi gian TP Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 18 -4.64056 X 30 18 -4.60278 XX 60 18 -4.56722 XX 90 18 -4.50611 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 30 -0.0377778 0.129579 0 - 60 -0.0733333 0.129579 0 - 90 *-0.134444 0.129579 30 - 60 -0.0355556 0.129579 30 - 90 -0.0966667 0.129579 60 - 90 -0.0611111 0.129579 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for a by Nhiet Do TP.so -------------------------------------------------------------------------------- Method: 95.0 percent LSD Nhiet Do TP.so Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 54 36 -4.60056 X 64 36 -4.55778 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 54 - 64 -0.0427778 0.0916258 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for a by Mau Thi Nghiem.TP Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 0.207678 6 0.034613 0.88 0.5113 Within groups 2.54307 65 0.0391242 ----------------------------------------------------------------------------- Total (Corr.) 2.75075 71 xxiv Multiple Range Tests for a by Mau Thi Nghiem.TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- DC 18 -4.64056 X 62-65/30 9 -4.61111 XX 52-55/30 9 -4.59444 XX 52-55/60 9 -4.58111 XX 62-65/60 9 -4.55333 XX 52-55/90 9 -4.54444 XX 62-65/90 9 -4.46778 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 -0.0133333 0.18622 52-55/30 - 52-55/90 -0.05 0.18622 52-55/30 - 62-65/30 0.0166667 0.18622 52-55/30 - 62-65/60 -0.0411111 0.18622 52-55/30 - 62-65/90 -0.126667 0.18622 52-55/30 - DC 0.0461111 0.161271 52-55/60 - 52-55/90 -0.0366667 0.18622 52-55/60 - 62-65/30 0.03 0.18622 52-55/60 - 62-65/60 -0.0277778 0.18622 52-55/60 - 62-65/90 -0.113333 0.18622 52-55/60 - DC 0.0594444 0.161271 52-55/90 - 62-65/30 0.0666667 0.18622 52-55/90 - 62-65/60 0.00888889 0.18622 52-55/90 - 62-65/90 -0.0766667 0.18622 52-55/90 - DC 0.0961111 0.161271 62-65/30 - 62-65/60 -0.0577778 0.18622 62-65/30 - 62-65/90 -0.143333 0.18622 62-65/30 - DC 0.0294444 0.161271 62-65/60 - 62-65/90 -0.0855556 0.18622 62-65/60 - DC 0.0872222 0.161271 62-65/90 - DC *0.172778 0.161271 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Analysis of Variance for b - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Thoi gian TP 0.251878 3 0.0839593 1.58 0.2027 B:Nhiet Do TP.so 0.08405 1 0.08405 1.58 0.2131 RESIDUAL 3.56372 67 0.0531898 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 3.89964 71 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. xxv Multiple Range Tests for b by Thoi gian TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Thoi gian TP Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 90 18 14.3994 X 60 18 14.4844 X 30 18 14.5389 X 0 18 14.5483 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 30 0.00944444 0.153446 0 - 60 0.0638889 0.153446 0 - 90 0.148889 0.153446 30 - 60 0.0544444 0.153446 30 - 90 0.139444 0.153446 60 - 90 0.085 0.153446 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for b by Nhiet Do TP.so -------------------------------------------------------------------------------- Method: 95.0 percent LSD Nhiet Do TP.so Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 64 36 14.4586 X 54 36 14.5269 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 54 - 64 0.0683333 0.108503 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for b by Mau Thi Nghiem.TP Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 0.333661 6 0.0556102 1.01 0.4243 Within groups 3.56598 65 0.0548613 ----------------------------------------------------------------------------- Total (Corr.) 3.89964 71 xxvi Multiple Range Tests for b by Mau Thi Nghiem.TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 62-65/90 9 14.3422 X 62-65/60 9 14.4489 XX 52-55/90 9 14.4567 XX 52-55/60 9 14.52 XX 62-65/30 9 14.5367 XX 52-55/30 9 14.5411 XX DC 18 14.5483 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 0.0211111 0.220514 52-55/30 - 52-55/90 0.0844444 0.220514 52-55/30 - 62-65/30 0.00444444 0.220514 52-55/30 - 62-65/60 0.0922222 0.220514 52-55/30 - 62-65/90 0.198889 0.220514 52-55/30 - DC -0.00722222 0.19097 52-55/60 - 52-55/90 0.0633333 0.220514 52-55/60 - 62-65/30 -0.0166667 0.220514 52-55/60 - 62-65/60 0.0711111 0.220514 52-55/60 - 62-65/90 0.177778 0.220514 52-55/60 - DC -0.0283333 0.19097 52-55/90 - 62-65/30 -0.08 0.220514 52-55/90 - 62-65/60 0.00777778 0.220514 52-55/90 - 62-65/90 0.114444 0.220514 52-55/90 - DC -0.0916667 0.19097 62-65/30 - 62-65/60 0.0877778 0.220514 62-65/30 - 62-65/90 0.194444 0.220514 62-65/30 - DC -0.0116667 0.19097 62-65/60 - 62-65/90 0.106667 0.220514 62-65/60 - DC -0.0994444 0.19097 62-65/90 - DC *-0.206111 0.19097 -------------------------------------------------------------------------------- * denotes a statistically significant difference. b. Ảnh hưởng của quá trình thủy phân tinh bột bắp đến độ nhớt sản phẩm Analysis of Variance for Do Nhot - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Thoi gian TP 101.989 3 33.9962 542.25 0.0000 B:Nhiet Do TP.so 1.74533 1 1.74533 27.84 0.0000 RESIDUAL 4.20053 67 0.0626944 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 107.934 71 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. xxvii Multiple Range Tests for Do Nhot by Thoi gian TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Thoi gian TP Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 90 18 2.43778 X 60 18 2.96944 X 30 18 3.56722 X 0 18 5.58056 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 30 *2.01333 0.166593 0 - 60 *2.61111 0.166593 0 - 90 *3.14278 0.166593 30 - 60 *0.597778 0.166593 30 - 90 *1.12944 0.166593 60 - 90 *0.531667 0.166593 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for Do Nhot by Nhiet Do TP.so -------------------------------------------------------------------------------- Method: 95.0 percent LSD Nhiet Do TP.so Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 64 36 3.48306 X 54 36 3.79444 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 54 - 64 *0.311389 0.117799 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for Do Nhot by Mau Thi Nghiem.TP Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 104.89 6 17.4817 373.25 0.0000 Within groups 3.04436 65 0.0468363 ----------------------------------------------------------------------------- Total (Corr.) 107.934 71 xxviii Multiple Range Tests for Do Nhot by Mau Thi Nghiem.TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 62-65/90 9 2.23222 X 52-55/90 9 2.64333 X 62-65/60 9 2.64889 X 52-55/60 9 3.29 X 62-65/30 9 3.44 X 52-55/30 9 3.69444 X DC 18 5.58056 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 *0.404444 0.203748 52-55/30 - 52-55/90 *1.05111 0.203748 52-55/30 - 62-65/30 *0.254444 0.203748 52-55/30 - 62-65/60 *1.04556 0.203748 52-55/30 - 62-65/90 *1.46222 0.203748 52-55/30 - DC *-1.88611 0.176451 52-55/60 - 52-55/90 *0.646667 0.203748 52-55/60 - 62-65/30 -0.15 0.203748 52-55/60 - 62-65/60 *0.641111 0.203748 52-55/60 - 62-65/90 *1.05778 0.203748 52-55/60 - DC *-2.29056 0.176451 52-55/90 - 62-65/30 *-0.796667 0.203748 52-55/90 - 62-65/60 -0.00555556 0.203748 52-55/90 - 62-65/90 *0.411111 0.203748 52-55/90 - DC *-2.93722 0.176451 62-65/30 - 62-65/60 *0.791111 0.203748 62-65/30 - 62-65/90 *1.20778 0.203748 62-65/30 - DC *-2.14056 0.176451 62-65/60 - 62-65/90 *0.416667 0.203748 62-65/60 - DC *-2.93167 0.176451 62-65/90 - DC *-3.34833 0.176451 -------------------------------------------------------------------------------- * denotes a statistically significant difference. 2.1.2 Ảnh hưởng của chế độ thủy phân tinh bột bắp đến lượng đường sinh của dịch thủy phân Analysis of Variance for Ham Luong Duong - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Thoi gian TP 2848.37 3 949.456 659.89 0.0000 B:Nhiet Do TP.so 207.027 1 207.027 143.89 0.0000 RESIDUAL 96.3999 67 1.4388 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 3151.8 71 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. xxix Multiple Range Tests for Ham Luong Duong by Thoi gian TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Thoi gian TP Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 18 2.63333 X 30 18 13.2511 X 60 18 16.4739 X 90 18 19.22 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 30 *-10.6178 0.798073 0 - 60 *-13.8406 0.798073 0 - 90 *-16.5867 0.798073 30 - 60 *-3.22278 0.798073 30 - 90 *-5.96889 0.798073 60 - 90 *-2.74611 0.798073 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for Ham Luong Duong by Nhiet Do TP.so -------------------------------------------------------------------------------- Method: 95.0 percent LSD Nhiet Do TP.so Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 54 36 11.1989 X 64 36 14.5903 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 54 - 64 *-3.39139 0.564323 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for Ham Luong Duong by Nhiet Do TP.so -------------------------------------------------------------------------------- Method: 95.0 percent LSD Nhiet Do TP.so Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 54 36 11.1989 X 64 36 14.5903 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 54 - 64 *-3.39139 0.564323 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for Ham Luong Duong by Mau Thi Nghiem.TP Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 3148.32 6 524.72 9809.63 0.0000 Within groups 3.47687 65 0.0534903 ----------------------------------------------------------------------------- Total (Corr.) 3151.8 71 xxx Multiple Range Tests for Ham Luong Duong by Mau Thi Nghiem.TP -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- DC 18 2.63333 X 52-55/30 9 11.8933 X 52-55/60 9 13.5322 X 62-65/30 9 14.6089 X 52-55/90 9 16.7367 X 62-65/60 9 19.4156 X 62-65/90 9 21.7033 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 *-1.63889 0.217741 52-55/30 - 52-55/90 *-4.84333 0.217741 52-55/30 - 62-65/30 *-2.71556 0.217741 52-55/30 - 62-65/60 *-7.52222 0.217741 52-55/30 - 62-65/90 *-9.81 0.217741 52-55/30 - DC *9.26 0.188569 52-55/60 - 52-55/90 *-3.20444 0.217741 52-55/60 - 62-65/30 *-1.07667 0.217741 52-55/60 - 62-65/60 *-5.88333 0.217741 52-55/60 - 62-65/90 *-8.17111 0.217741 52-55/60 - DC *10.8989 0.188569 52-55/90 - 62-65/30 *2.12778 0.217741 52-55/90 - 62-65/60 *-2.67889 0.217741 52-55/90 - 62-65/90 *-4.96667 0.217741 52-55/90 - DC *14.1033 0.188569 62-65/30 - 62-65/60 *-4.80667 0.217741 62-65/30 - 62-65/90 *-7.09444 0.217741 62-65/30 - DC *11.9756 0.188569 62-65/60 - 62-65/90 *-2.28778 0.217741 62-65/60 - DC *16.7822 0.188569 62-65/90 - DC *19.07 0.188569 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xxxi 2.1.3 Ảnh hưởng của chế độ thủy phân tinh bột bắp đến giá trị cảm quan sản phẩm Analysis of Variance for Trang thai san pham - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Thoi Gian. Cam Q 535.8 3 178.6 776.28 0.0000 B:Nhiet Do. CQ. so 45.0667 1 45.0667 195.88 0.0000 RESIDUAL 54.0667 235 0.230071 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 634.933 239 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. Multiple Range Tests for Trang thai san pham by Thoi Gian. Cam Quan -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 60 0.55 X 30 60 2.35 X 60 60 3.65 X 90 60 4.51667 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 30 *-1.8 0.172529 0 - 60 *-3.1 0.172529 0 - 90 *-3.96667 0.172529 30 - 60 *-1.3 0.172529 30 - 90 *-2.16667 0.172529 60 - 90 *-0.866667 0.172529 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Multiple Range Tests for Trang thai san pham by Nhiet Do. Cam Quan -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 52-55 120 2.33333 X 62-65 120 3.2 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55 - 62-65 *-0.866667 0.121996 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for Trang thai san pham by Mau Thi Nghiem. CQ Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 595.717 6 99.2861 589.89 0.0000 Within groups 39.2167 233 0.168312 ----------------------------------------------------------------------------- Total (Corr.) 634.933 239 xxxii Multiple Range Tests for Trang thai san pham by Mau Thi Nghiem. CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- DC 60 0.55 X 52-55/30 30 1.6 X 62-65/30 30 3.1 X 52-55/60 30 3.16667 X 52-55/90 30 4.06667 X 62-65/60 30 4.13333 X 62-65/90 30 4.96667 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 52-55/30 - 52-55/60 *-1.56667 0.2087 52-55/30 - 52-55/90 *-2.46667 0.2087 52-55/30 - 62-65/30 *-1.5 0.2087 52-55/30 - 62-65/60 *-2.53333 0.2087 52-55/30 - 62-65/90 *-3.36667 0.2087 52-55/30 - DC *1.05 0.180739 52-55/60 - 52-55/90 *-0.9 0.2087 52-55/60 - 62-65/30 0.0666667 0.2087 52-55/60 - 62-65/60 *-0.966667 0.2087 52-55/60 - 62-65/90 *-1.8 0.2087 52-55/60 - DC *2.61667 0.180739 52-55/90 - 62-65/30 *0.966667 0.2087 52-55/90 - 62-65/60 -0.0666667 0.2087 52-55/90 - 62-65/90 *-0.9 0.2087 52-55/90 - DC *3.51667 0.180739 62-65/30 - 62-65/60 *-1.03333 0.2087 62-65/30 - 62-65/90 *-1.86667 0.2087 62-65/30 - DC *2.55 0.180739 62-65/60 - 62-65/90 *-0.833333 0.2087 62-65/60 - DC *3.58333 0.180739 62-65/90 - DC *4.41667 0.180739 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xxxiii 2.2 Ảnh hưởng của tỷ lệ phối chế dịch bắp thủy phân và sữa đến chất lượng sản phẩm 2.2.1 Ảnh hưởng của tỷ lệ phối chế dịch bắp thủy phân và sữa đến đặc tính vật lý sản phẩm a. Ảnh hưởng của tỷ lệ phối chế dịch bắp thủy phân và sữa đến giá trị L,a, b của sản phẩm ANOVA Table for L by Ty Le Bap Bo Sung Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 470.978 3 156.993 404.58 0.0000 Within groups 12.4171 32 0.388035 ----------------------------------------------------------------------------- Total (Corr.) 483.395 35 Multiple Range Tests for L by Ty Le Bap Bo Sung -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 3 9 75.0256 X 2 9 78.4267 X 1 9 80.4567 X 0 9 85.0189 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *4.56222 0.598145 0 - 2 *6.59222 0.598145 0 - 3 *9.99333 0.598145 1 - 2 *2.03 0.598145 1 - 3 *5.43111 0.598145 2 - 3 *3.40111 0.598145 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for a by Ty Le Dich Bap Bo Sung Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 48.0715 3 16.0238 161.83 0.0000 Within groups 3.16858 32 0.0990181 ----------------------------------------------------------------------------- Total (Corr.) 51.2401 35 Multiple Range Tests for a by Ty Le Dich Bap Bo Sung -------------------------------------------------------------------------------- Method: 95.0 percent LSD Mau thi nghiem Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 3 9 -5.65333 X 2 9 -4.54111 X 1 9 -3.56889 X 0 9 -2.53333 X -------------------------------------------------------------------------------- xxxiv ANOVA Table for b by Ty Le Bap Bo Sung Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 1000.06 3 333.352 2843.12 0.0000 Within groups 3.75196 32 0.117249 ----------------------------------------------------------------------------- Total (Corr.) 1003.81 35 Multiple Range Tests for b by Ty Le Bap Bo Sung -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 9 2.77333 X 1 9 10.5533 X 2 9 14.4022 X 3 9 16.63 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *-7.78 0.328795 0 - 2 *-11.6289 0.328795 0 - 3 *-13.8567 0.328795 1 - 2 *-3.84889 0.328795 1 - 3 *-6.07667 0.328795 2 - 3 *-2.22778 0.328795 -------------------------------------------------------------------------------- * denotes a statistically significant difference. b. Ảnh hưởng của tỷ lệ phối chế dịch bắp thủy phân và sữa đến độ nhớt của sản phẩm ANOVA Table for Do Nhot by Ty Le Bap Bo Sung Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 3.66114 3 1.22038 365.20 0.0000 Within groups 0.106933 32 0.00334167 ----------------------------------------------------------------------------- Total (Corr.) 3.76807 35 ANOVA Table for Do Nhot by Ty Le Bap Bo Sung Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 3.66114 3 1.22038 365.20 0.0000 Within groups 0.106933 32 0.00334167 ----------------------------------------------------------------------------- Total (Corr.) 3.76807 35 xxxv Multiple Range Tests for Do Nhot by Ty Le Bap Bo Sung -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 9 1.80667 X 1 9 2.12222 X 2 9 2.32222 X 3 9 2.68556 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *-0.315556 0.0555077 0 - 2 *-0.515556 0.0555077 0 - 3 *-0.878889 0.0555077 1 - 2 *-0.2 0.0555077 1 - 3 *-0.563333 0.0555077 2 - 3 *-0.363333 0.0555077 -------------------------------------------------------------------------------- * denotes a statistically significant difference. 2.2.2 Ảnh hưởng của tỷ lệ phối chế dịch bắp thủy phân và sữa đến giá trị cảm quan Analysis of Variance for Mau - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Ty Le Bap Bo Sun 175.833 3 58.6111 670.94 0.0000 RESIDUAL 10.1333 116 0.0873563 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 185.967 119 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. Multiple Range Tests for Mau by Ty Le Bap Bo Sung.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 30 2.0 X 1 30 3.06667 X 3 30 4.8 X 2 30 4.86667 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *-1.06667 0.151149 0 - 2 *-2.86667 0.151149 0 - 3 *-2.8 0.151149 1 - 2 *-1.8 0.151149 1 - 3 *-1.73333 0.151149 2 - 3 0.0666667 0.151149 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xxxvi ANOVA Table for Mui by Ty Le Bap Bo Sung.CQ Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 156.9 3 52.3 669.13 0.0000 Within groups 9.06667 116 0.0781609 ----------------------------------------------------------------------------- Total (Corr.) 165.967 119 Multiple Range Tests for Mui by Ty Le Bap Bo Sung.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 3 30 2.06667 X 0 30 2.06667 X 1 30 3.06667 X 2 30 4.86667 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *-1.0 0.142973 0 - 2 *-2.8 0.142973 0 - 3 0.0 0.142973 1 - 2 *-1.8 0.142973 1 - 3 *1.0 0.142973 2 - 3 *2.8 0.142973 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for Vi by Ty Le Bap Bo Sung.CQ Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 117.825 3 39.275 256.43 0.0000 Within groups 17.7667 116 0.153161 ----------------------------------------------------------------------------- Total (Corr.) 135.592 119 xxxvii Multiple Range Tests for Vi by Ty Le Bap Bo Sung.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 0 30 2.0 X 1 30 3.3 X 3 30 3.33333 X 2 30 4.8 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 0 - 1 *-1.3 0.200139 0 - 2 *-2.8 0.200139 0 - 3 *-1.33333 0.200139 1 - 2 *-1.5 0.200139 1 - 3 -0.0333333 0.200139 2 - 3 *1.46667 0.200139 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xxxviii 2.3 Ảnh hưởng của chế độ tiệt trùng đến chất lượng và bảo quản sản phẩm 2.3.1 Ảnh hưởng của chế độ tiệt trùng đến đặc tính vật lý sản phẩm a. Ảnh hưởng của chế độ tiệt trùng đến Giá trị L,a,b sản phẩm ANOVA Table for L by Mau thi nghiem Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 184.427 3 61.4758 117.34 0.0000 Within groups 16.7656 32 0.523925 ----------------------------------------------------------------------------- Total (Corr.) 201.193 35 Multiple Range Tests for L by Mau thi nghiem -------------------------------------------------------------------------------- Method: 95.0 percent LSD Mau thi nghiem Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/20 9 73.0767 X 125/10 9 76.6733 X 125/5 9 78.4367 X 121/15 9 78.7867 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *5.71 0.695034 121/15 - 125/10 *2.11333 0.695034 121/15 - 125/5 0.35 0.695034 121/20 - 125/10 *-3.59667 0.695034 121/20 - 125/5 *-5.36 0.695034 125/10 - 125/5 *-1.76333 0.695034 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for a by Mau thi nghiem Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 45.6246 3 15.2082 745.80 0.0000 Within groups 0.652533 32 0.0203917 ----------------------------------------------------------------------------- Total (Corr.) 46.2771 35 xxxix Multiple Range Tests for a by Mau thi nghiem -------------------------------------------------------------------------------- Method: 95.0 percent LSD Mau thi nghiem Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/20 9 -7.04889 X 125/10 9 -5.71444 X 125/5 9 -4.36556 X 121/15 9 -4.31 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *2.73889 0.137119 121/15 - 125/10 *1.40444 0.137119 121/15 - 125/5 0.0555556 0.137119 121/20 - 125/10 *-1.33444 0.137119 121/20 - 125/5 *-2.68333 0.137119 125/10 - 125/5 *-1.34889 0.137119 -------------------------------------------------------------------------------- * denotes a statistically significant difference. ANOVA Table for b by Mau thi nghiem Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 113.698 3 37.8994 418.02 0.0000 Within groups 2.90124 32 0.0906639 ----------------------------------------------------------------------------- Total (Corr.) 116.6 35 Multiple Range Tests for b by Mau thi nghiem -------------------------------------------------------------------------------- Method: 95.0 percent LSD Mau thi nghiem Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/15 9 14.4889 X 125/5 9 14.4922 X 125/10 9 16.4633 X 121/20 9 18.8067 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *-4.31778 0.289127 121/15 - 125/10 *-1.97444 0.289127 121/15 - 125/5 -0.00333333 0.289127 121/20 - 125/10 *2.34333 0.289127 121/20 - 125/5 *4.31444 0.289127 125/10 - 125/5 *1.97111 0.289127 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xl b. Ảnh hưởng của chế độ tiệt trùng đến độ nhớt sản phẩm ANOVA Table for Do nhot by Mau thi nghiem Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 0.0160333 3 0.00534444 2.00 0.1339 Within groups 0.0855556 32 0.00267361 ----------------------------------------------------------------------------- Total (Corr.) 0.101589 35 Multiple Range Tests for Do nhot by Mau thi nghiem -------------------------------------------------------------------------------- Method: 95.0 percent LSD Mau thi nghiem Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/20 9 2.24222 X 125/10 9 2.25556 X 125/5 9 2.28889 X 121/15 9 2.29111 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 0.0488889 0.0496501 121/15 - 125/10 0.0355556 0.0496501 121/15 - 125/5 0.00222222 0.0496501 121/20 - 125/10 -0.0133333 0.0496501 121/20 - 125/5 -0.0466667 0.0496501 125/10 - 125/5 -0.0333333 0.0496501 -------------------------------------------------------------------------------- * denotes a statistically significant difference. 2.3.2 Ảnh hưởng của chế độ tiệt trùng đến mật số vi sinh vật và khả năng bảo quản Multiple Range Tests for So khuan lac by Mau thi nghiem.vi sinh -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/20 6 0.0 X 125/10 6 0.0 X 125/5 6 0.0 X 121/15 6 0.0 X -------------------------------------------------------------------------------- 2.3.3 Ảnh hưởng của chế độ tiệt trùng đến giá tri cảm quan sản phẩm Analysis of Variance for Mau - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Mau thi nghiem.C 273.9 3 91.3 432.87 0.0000 RESIDUAL 24.4667 116 0.21092 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 298.367 119 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. xli Multiple Range Tests for Mau by Mau thi nghiem.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 121/20 30 1.4 X 125/10 30 2.2 X 121/15 30 4.7 X 125/5 30 4.83333 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *3.3 0.234864 121/15 - 125/10 *2.5 0.234864 121/15 - 125/5 -0.133333 0.234864 121/20 - 125/10 *-0.8 0.234864 121/20 - 125/5 *-3.43333 0.234864 125/10 - 125/5 *-2.63333 0.234864 -------------------------------------------------------------------------------- * denotes a statistically significant difference. Analysis of Variance for Mui - Type III Sums of Squares -------------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value -------------------------------------------------------------------------------- MAIN EFFECTS A:Mau thi nghiem.C 209.425 3 69.8083 234.27 0.0000 RESIDUAL 34.5667 116 0.297989 -------------------------------------------------------------------------------- TOTAL (CORRECTED) 243.992 119 -------------------------------------------------------------------------------- All F-ratios are based on the residual mean square error. Multiple Range Tests for Mui by Mau thi nghiem.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count LS Mean Homogeneous Groups -------------------------------------------------------------------------------- 125/10 30 1.5 X 121/20 30 2.2 X 121/15 30 3.8 X 125/5 30 4.86667 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *1.6 0.279163 121/15 - 125/10 *2.3 0.279163 121/15 - 125/5 *-1.06667 0.279163 121/20 - 125/10 *0.7 0.279163 121/20 - 125/5 *-2.66667 0.279163 125/10 - 125/5 *-3.36667 0.279163 -------------------------------------------------------------------------------- * denotes a statistically significant difference. xlii ANOVA Table for Vi by Mau thi nghiem.CQ Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Between groups 303.492 3 101.164 336.89 0.0000 Within groups 34.8333 116 0.300287 ----------------------------------------------------------------------------- Total (Corr.) 338.325 119 Multiple Range Tests for Vi by Mau thi nghiem.CQ -------------------------------------------------------------------------------- Method: 95.0 percent LSD Level Count Mean Homogeneous Groups -------------------------------------------------------------------------------- 125/10 30 1.26667 X 121/20 30 1.43333 X 121/15 30 4.2 X 125/5 30 4.8 X -------------------------------------------------------------------------------- Contrast Difference +/- Limits -------------------------------------------------------------------------------- 121/15 - 121/20 *2.76667 0.280237 121/15 - 125/10 *2.93333 0.280237 121/15 - 125/5 *-0.6 0.280237 121/20 - 125/10 0.166667 0.280237 121/20 - 125/5 *-3.36667 0.280237 125/10 - 125/5 *-3.53333 0.280237 -------------------------------------------------------------------------------- * denotes a statistically significant difference. PHỤ LỤC 3 ĐỒ THỊ 3.1Vẽ đồ thị thí nghiệm 1 3.1.1 Hàm lượng đường theo nhiệt độ và thời gian Ham Luong Duong = 2.20653 + 0.0194715*Thoi gian TP - 0.0953687*Nhiet Do TP.so - 0.000176543*Thoi gian TP*Thoi gian TP + 0.00376078*Nhiet Do TP.so*Nhiet Do TP.so + 0.0017297*Thoi gian TP*Nhiet Do TP.so xliii Multiple Regression Analysis ----------------------------------------------------------------------------- Dependent variable: Ham Luong Duong ----------------------------------------------------------------------------- Standard T Parameter Estimate Error Statistic P-Value ----------------------------------------------------------------------------- CONSTANT 2.20653 0.479608 4.60068 0.0000 Thoi gian TP 0.0194715 0.0166174 1.17175 0.2450 Nhiet Do TP.so -0.0953687 0.0155728 -6.12407 0.0000 Thoi gian TP*Thoi -0.000176543 0.000134399 -1.31357 0.1930 Nhiet Do TP.so*Nh 0.00376078 0.000240999 15.605 0.0000 Thoi gian TP*Nhie 0.0017297 0.00008281 20.8875 0.0000 ----------------------------------------------------------------------------- Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Model 3974.54 5 794.908 3018.33 0.0000 Residual 19.752 75 0.263361 ----------------------------------------------------------------------------- Total (Corr.) 3994.29 80 R-squared = 99.5055 percent R-squared (adjusted for d.f.) = 99.4725 percent Standard Error of Est. = 0.513187 Mean absolute error = 0.372185 Durbin-Watson statistic = 0.686511 3.1.2 Độ nhớt xliv Multiple Regression Analysis ----------------------------------------------------------------------------- Dependent variable: Do Nhot ----------------------------------------------------------------------------- Standard T Parameter Estimate Error Statistic P-Value ----------------------------------------------------------------------------- CONSTANT 5.56551 0.211951 26.2585 0.0000 Thoi gian TP 0.000478197 0.00734364 0.0651172 0.9483 Nhiet Do TP.so -0.0243324 0.006882 -3.53566 0.0007 Thoi gian TP*Thoi -0.00000699588 0.0000593944 -0.117787 0.9066 Nhiet Do TP.so*Nh -0.0000151749 0.000106504 -0.142482 0.8871 Thoi gian TP*Nhie -0.000307826 0.0000365958 -8.41151 0.0000 ----------------------------------------------------------------------------- Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Model 133.558 5 26.7116 519.34 0.0000 Residual 3.85753 75 0.0514337 ----------------------------------------------------------------------------- Total (Corr.) 137.416 80 R-squared = 97.1928 percent R-squared (adjusted for d.f.) = 97.0057 percent Standard Error of Est. = 0.22679 Mean absolute error = 0.157463 Durbin-Watson statistic = 1.77435 Do Nhot = 5.56551 + 0.000478197*Thoi gian TP - 0.0243324*Nhiet Do TP.so - 0.00000699588*Thoi gian TP*Thoi gian TP - 0.0000151749*Nhiet Do TP.so*Nhiet Do TP.so - 0.000307826*Thoi gian TP*Nhiet Do TP.so 3.1.3 Giá trị L, a, b xlv L = 79.0628 - 0.00457557*Thoi gian TP + 0.00483276*Nhiet Do TP.so + 0.0000393004*Thoi gian TP*Thoi gian TP - 0.000105346*Nhiet Do TP.so*Nhiet Do TP.so - 0.0000708974*Thoi gian TP*Nhiet Do TP.so Multiple Regression Analysis ----------------------------------------------------------------------------- Dependent variable: L ----------------------------------------------------------------------------- Standard T Parameter Estimate Error Statistic P-Value ----------------------------------------------------------------------------- CONSTANT 79.0628 0.312636 252.891 0.0000 Thoi gian TP -0.00457557 0.0108322 -0.422405 0.6739 Nhiet Do TP.so 0.00483276 0.0101512 0.476076 0.6354 Thoi gian TP*Thoi 0.0000393004 0.0000876092 0.448588 0.6550 Nhiet Do TP.so*Nh -0.000105346 0.000157097 -0.670575 0.5046 Thoi gian TP*Nhie -0.0000708974 0.0000539803 -1.31339 0.1931 ----------------------------------------------------------------------------- Analysis of Variance ----------------------------------------------------------------------------- Source Sum of Squares Df Mean Square F-Ratio P-Value ----------------------------------------------------------------------------- Model 2.76829 5 0.553658 4.95 0.0006 Residual 8.39301 75 0.111907 ----------------------------------------------------------------------------- Total (Corr.) 11.1613 80 R-squared = 95.8026 percent R-squared (adjusted for d.f.) = 19.7894 percent Standard Error of Est. = 0.334525 Mean absolute error = 0.253022 Durbin-Watson statistic = 1.34189

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