A new synthesis process of lignosulfonate using lignin recovered from black liquor of pulp and paper mills - Nguyen Truong GiangLignosulfonate was successfully synthesized via formaldehyde and sodium sulfite. FT-IR spectrum of both of lignosulfonates appeared peak at 626.9 cm-1, corresponds to (C=S) linkage of the sulfonic acid group. Surface tension of LS solutions are 46 mN/m and 44 mN/m, respectively. Two steps methyl sulfonation of lignin performed at 85 ºC, for 90 ...
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Expression of glutaryl7-Aminocephalosporanic acid acylase in escherichia coli bl21(de3) and immobilization of recombinant enzyme on nanoporous materials - Vu Thi Hanh NguyenBy expression of gla gene in E. coli BL21(DE3), the recombinant GLA was successfully synthesized in LB medium at 30 oC and induced by 0.4 mM of IPTG for 3 hours. Biochemical analysis showed that the recombinant enzyme has molecular weight of about 83 kDa and specific activity of 2.7 U/mg of protein. Among of nanoporous materials for immobilizati...
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Production of polyclonal antibodies against recombinant vp6 antigen of rotavirus - Do Thi Thu HaThe present study has successfully produced pure recombinant rotavirus VP6 protein. The recombinant VP6 protein was highly immunogenic in rabbits. The generated polyclonal antibody was specific to the VP6 protein of rotavirus and could be used to the development of lateral flow immunoassay test trip for detection of rotavirus.
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Covalent conjugation of antibody and gold nanoparticle for development of lateral flow immunoassay test strip - Truong Quoc PhongAs can be seen in Fig. 5A, the strongest signal band is observed for the coupling reaction at pH 4.0. Figure 5B shows that the most effective coupling conditions are observed for 0.01 mM of EDC/NHS. The coupling yield seems to decrease similarly by increasing or decreasing the concentrations of EDC and NHS. Bartczak et al., 2011 also showed that...
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Improvement of lycopene extraction from tomatoes by enzyme – assisted treatment - Vu Phuong NamThe results of this study indicated that the lycopene extraction form tomatoes can be greatly enhanced by pretreatment of tomato purée by pectinase (Pectinex Ultra Clear) and cellulase enzymes (Cellulas 1.5L and Viscozyme L). The optimum conditions for enzymatic treatment of tomato purée by Pectinex Ultra Clear for lycopene extraction were deter...
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Study on the use of acidophilic iron oxidizing bacteria for dissolving iron from low-Graded chalcopyrite oresIn this study, two strains of acidophilic iron oxidizing bacteria Acidithiobacillus sp. IOB1 and Leptospirillum sp. IOB2 originated from mining sites in Vietnam were used as starting cultures for bioleaching experiments in the laboratory. These strains showed high capability of promoting metal solubilization from low-graded chalcopyrite ore, esp...
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Research on protein hydrolysis from shrimp waste using commercial proteases - TranThi LinhCombination of Alcalase and Flavouzyme is needed to improve the protein hydrolysis. The time of addition Flavourzyme seemed important. The simultaneous or sequential mode of adding Flavourzyme affected the protein recovery yield as well as aminoacid yield. Whereas simultaneous adding of Flavourzyme could improve the aminoacid recovery yieldbut n...
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Cellulose nanocrystals: synthesis, characteristics and effect on hydroxypropyl methylcellulose-Based composite films and coatings - Nguyen Thi Minh NguyetMục đích của nghiên cứu là tổng hợp được cellulose nanocrystals, xác định được các đặc trưng cơ bản như kích thước hạt, phân bố hạt, thế zeta, phổ FT-IR và TEM, đồng thời có được đánh giá về tác dụng của loại nano này khi bổ sung vào composite HPMC có chứa nano nhũ tương sáp carnauba và nano chitosan tới một số đăc trưng của màng film và màng ph...
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Recovery of pectic oligosaccharide (pos) from pectin hydolysate for funtional foods - Nguyen Thi Kim DungPectic oligosaccharide (POS), sản phẩm thủy phân không hoàn toàn của pectin, là một prebiotic thế hệ mới có nhiều đặc tính quý có lợi cho sức khỏe của người và vật nuôi. Hiện tại mới chỉ có một số công bố về quy trình sản xuất POS ở quy mô phòng thí nghiệm và pilot, tuy nhiên công nghệ sản xuất và sản phẩm vẫn chưa được chào bán trên thị trường....
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Effect of ultrasound on pretreatment of tuna skin for gelatin production - Dang Minh NhatTable 1 showed the difference in some characteristics of two samples produced from two methods, especially in lipid content and gel strength. The traditional method using lime for pretreatment gave gelatin with rather high lipid content in comparison with the new method also using lime for pretreatment but with ultrasound assistance (1.81 % comp...
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